Assessment of the Aging of the Brown Adipose Tissue by 18F-FDG PETCT Imaging in the Progeria Mouse Model Lmna−−
Joint Authors
Wang, Jing
Kang, Fei
Wang, Zhengjie
Xu, Xiaolong
Liu, Yi
Gao, Yongheng
Liu, Baohua
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Brown adipose tissue (BAT) is an important energy metabolic organ that is highly implicated in obesity, type 2 diabetes, and atherosclerosis.
Aging is one of the most important determinants of BAT activity.
In this study, we used 18F-FDG PET/CT imaging to assess BAT aging in Lmna−/− mice.
The maximum standardized uptake value (SUVMax) of the BAT was measured, and the target/nontarget (T/NT) values of BAT were calculated.
The transcription and the protein expression levels of the uncoupling protein 1 (UCP1), beta3-adrenergic receptor (β3-AR), and the PR domain-containing 16 (PRDM16) were measured by quantitative real-time polymerase chain reaction (RT-PCR) and Western blotting or immunohistochemical analysis.
Apoptosis and cell senescence rates in the BAT of WT and Lmna−/− mice were determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and by CDKN2A/p16INK4a immunohistochemical staining, respectively.
At 14 weeks of age, the BAT SUVMax and the expression levels of UCP1, β3-AR, and PRDM16 in Lmna−/− mice were significantly reduced relative to WT mice.
At the same time, the number of p16INK4a and TUNEL positively stained cells (%) increased in Lmna−/− mice.
Collectively, our results indicate that the aging characteristics and the aging regulatory mechanism in the BAT of Lmna−/− mice can mimic the normal BAT aging process.
American Psychological Association (APA)
Wang, Zhengjie& Xu, Xiaolong& Liu, Yi& Gao, Yongheng& Kang, Fei& Liu, Baohua…[et al.]. 2018. Assessment of the Aging of the Brown Adipose Tissue by 18F-FDG PETCT Imaging in the Progeria Mouse Model Lmna−−. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131575
Modern Language Association (MLA)
Wang, Zhengjie…[et al.]. Assessment of the Aging of the Brown Adipose Tissue by 18F-FDG PETCT Imaging in the Progeria Mouse Model Lmna−−. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1131575
American Medical Association (AMA)
Wang, Zhengjie& Xu, Xiaolong& Liu, Yi& Gao, Yongheng& Kang, Fei& Liu, Baohua…[et al.]. Assessment of the Aging of the Brown Adipose Tissue by 18F-FDG PETCT Imaging in the Progeria Mouse Model Lmna−−. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1131575
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131575