Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta-Defensin 118
Joint Authors
Luo, Junqiu
Luo, Yuheng
Zheng, Ping
Yu, Bing
Chen, Daiwen
Mao, Xiangbing
Lin, Qian
Xie, Kunhong
He, Jun
Yu, Jie
Yan, Hui
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
β-Defensin 118 (DEFB118) is a novel host defense peptide (HDP) identified in humans.
To evaluate its potentials for future utilization, the DEFB118 gene was expressed in Escherichia coli (E.
coli) and the recombinant protein was fully characterized.
Methods.
The DEFB118 protein was obtained by heterologous expression using E.
coli Rosetta (DE3).
Antibacterial activity of DEFB118 was determined by using various bacterial strains.
IPEC-J cells challenged by E.
coli K88 were used to determine its influences on inflammatory responses.
Results.
The E.
coli transformants yielded more than 250 μg/mL DEFB118 protein after 4 h induction by 1.0 mM IPTG.
The DEFB118 was estimated by SDS-PAGE to be 30 kDa, and MALDI-TOF analysis verified that it is a human β-defensin 118.
Importantly, the DEFB118 showed antimicrobial activities against both Gram-negative bacteria (E.
coli K88 and E.
coli DH5α) and Gram-positive bacteria (S.
aureus and B.
subtilis), with a minimum inhibitory concentration (MIC) of 4 μg/mL.
Hemolytic assays showed that DEFB118 had no detrimental impact on cell viability.
Additionally, DEFB118 was found to elevate the viability of IPEC-J2 cells upon E.
coli K88 challenge.
Moreover, DEFB118 significantly decreased cell apoptosis in the late apoptosis phase and downregulated the expression of inflammatory cytokines such as IL-1β and TNF-α in IPEC-J2 cell exposure to E.
coli K88.
Conclusions.
These results suggested a novel function of the mammalian defensins, and the antibacterial and anti-inflammatory properties of DEFB118 may allow it as a potential substitute for conventionally used antibiotics or drugs.
American Psychological Association (APA)
Lin, Qian& Xie, Kunhong& Chen, Daiwen& Yu, Bing& Mao, Xiangbing& Yu, Jie…[et al.]. 2020. Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta-Defensin 118. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1131625
Modern Language Association (MLA)
Lin, Qian…[et al.]. Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta-Defensin 118. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1131625
American Medical Association (AMA)
Lin, Qian& Xie, Kunhong& Chen, Daiwen& Yu, Bing& Mao, Xiangbing& Yu, Jie…[et al.]. Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta-Defensin 118. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1131625
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131625