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Detecting GPC3-Expressing Hepatocellular Carcinoma with L5 Peptide-Guided Pretargeting Approach: In Vitro and In Vivo MR Imaging Experiments
Joint Authors
Li, Weiyue
Xiao, Xiang
Li, Xiaodan
Xu, Yikai
Ma, Lichao
Guo, Liuji
Yan, Chenggong
Wu, Yuankui
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
To investigate the potential of L5 peptide-guided pretargeting approach to identify GPC3-expressing hepatocellular carcinoma (HCC) using ultrasmall superparamagnetic iron oxide (USPIO) as the MR probe.
Methods.
Immunofluorescence with carboxyfluorescein- (FAM-) labeled L5 peptide was performed in HepG2 cells.
Polyethylene glycol-modified USPIO (PEG-USPIO) and its conjugation with streptavidin (SA-PEG-USPIO) were synthesized, and their hydrodynamic diameters, zeta potential, T2 relaxivity, and cytotoxicity were measured.
In vitro and in vivo two-step pretargeting MR imaging was performed on HepG2 cells and tumor-bearing mice after the administration of biotinylated L5 peptide (first step), followed by SA-PEG-USPIO (second step).
Prussian blue staining was performed to assess iron deposition in tumors.
Results.
The high specificity of L5 peptide for GPC3 was demonstrated.
Generation of SA-PEG-USPIO nanoparticles with good biocompatibility (an average hydrodynamic diameter of 35.97 nm and a zeta potential of −7.91 mV), superparamagnetism (R2 = 0.1039 × 103 mM−1s−1), and low toxicity was achieved.
The pretargeting group showed more enhancement than the nonpretargeting group both in vitro (60% vs 20%, P<0.05) and in vivo (32% vs 6%, P<0.001).
Substantial iron deposition was only observed in HepG2 cells and tumors in the pretargeting group.
Conclusion.
L5 peptide-guided, two-step pretargeting approach with USPIO as the MR imaging probe is a lucrative strategy to specifically identify GPC3-expressing HCC.
American Psychological Association (APA)
Li, Weiyue& Xiao, Xiang& Li, Xiaodan& Xu, Yikai& Ma, Lichao& Guo, Liuji…[et al.]. 2018. Detecting GPC3-Expressing Hepatocellular Carcinoma with L5 Peptide-Guided Pretargeting Approach: In Vitro and In Vivo MR Imaging Experiments. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1131639
Modern Language Association (MLA)
Li, Weiyue…[et al.]. Detecting GPC3-Expressing Hepatocellular Carcinoma with L5 Peptide-Guided Pretargeting Approach: In Vitro and In Vivo MR Imaging Experiments. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1131639
American Medical Association (AMA)
Li, Weiyue& Xiao, Xiang& Li, Xiaodan& Xu, Yikai& Ma, Lichao& Guo, Liuji…[et al.]. Detecting GPC3-Expressing Hepatocellular Carcinoma with L5 Peptide-Guided Pretargeting Approach: In Vitro and In Vivo MR Imaging Experiments. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1131639
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131639