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Enhancement of Chemokine mRNA Expression by Toll-Like Receptor 2 Stimulation in Human Peripheral Blood Mononuclear Cells of Patients with Atopic Dermatitis
Joint Authors
Zhang, Wei
Yu, Bo
Yu, Yangyang
Lin, Dongxu
Cai, Xiaoqiong
Cui, Danni
Fang, Ran
Wang, Xiaomei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Atopic dermatitis (AD) is a chronic inflammatory skin disease which is often associated with Staphylococcus aureus (S.
aureus) colonization.
S.
aureus ingredients are potential ligands to activate the Toll-like receptor 2 (TLR2) and drive inflammatory cytokine or chemokine production.
However, the role of TLR2-mediated chemokine expression in AD development has not been systematically investigated.
In this study, we sought to determine the mode of TLR2-mediated chemokine expression in AD patients.
Human peripheral blood mononuclear cells (PBMCs) were isolated from AD patients and healthy controls.
Upon incubation with TLR2 ligands Pam3CSK4 and PGN, mRNA expression of chemokines, including CCL1, CCL5, CCL8, CCL13, CCL17, CCL18, CCL22, and CCL27, were determined by quantitative real-time polymerase chain reaction (qRT-PCR) analysis.
The results showed that basal mRNA expression of CCL17 in PBMCs from AD patients was upregulated compared with healthy controls, while those of CCL8 and CCL13 were downregulated.
When stimulated with TLR2 ligands, the mRNA expression of CCL5, CCL8, CCL13, CCL18, and CCL22 in PBMCs from AD patients was significantly higher than those from healthy controls.
The different basal chemokine mRNA expression profiles indicate the different immune status in patients with AD compared with healthy controls.
Excessive chemokine mRNA expression induced by TLR2 activation is associated with the development of AD.
American Psychological Association (APA)
Yu, Yangyang& Lin, Dongxu& Cai, Xiaoqiong& Cui, Danni& Fang, Ran& Zhang, Wei…[et al.]. 2020. Enhancement of Chemokine mRNA Expression by Toll-Like Receptor 2 Stimulation in Human Peripheral Blood Mononuclear Cells of Patients with Atopic Dermatitis. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1131691
Modern Language Association (MLA)
Yu, Yangyang…[et al.]. Enhancement of Chemokine mRNA Expression by Toll-Like Receptor 2 Stimulation in Human Peripheral Blood Mononuclear Cells of Patients with Atopic Dermatitis. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1131691
American Medical Association (AMA)
Yu, Yangyang& Lin, Dongxu& Cai, Xiaoqiong& Cui, Danni& Fang, Ran& Zhang, Wei…[et al.]. Enhancement of Chemokine mRNA Expression by Toll-Like Receptor 2 Stimulation in Human Peripheral Blood Mononuclear Cells of Patients with Atopic Dermatitis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1131691
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131691