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Identification of Key Genes and Long Noncoding RNA-Associated Competing Endogenous RNA (ceRNA) Networks in Early-Onset Preeclampsia
Joint Authors
Zhang, Zhan
Wang, Ping
Zhang, Linlin
Huang, Chenxi
Gao, Junjun
Li, Yingying
Yang, Bo
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Preeclampsia (PE) is a pregnancy-specific hypertension syndrome and is one of the leading causes of maternal and perinatal morbidity and mortality.
Long noncoding RNAs (lncRNAs) have been reported to be abnormally expressed in many diseases, including preeclampsia.
The present study is aimed at identifying the key genes and lncRNA-associated competing endogenous RNA (ceRNA) networks in early-onset preeclampsia (EOPE).
Methods.
We investigated expression profiles of differentially expressed lncRNAs (DElncRNAs) and genes (DEGs) in placental tissues of EOPE and healthy controls with Human LncRNA Array v4.
The potential functions of DEGs and DElncRNAs were predicted using the clusterProfiler package.
The lncRNA-mRNA coexpression network was constructed via Pearson’s correlation coefficient.
The protein-protein interaction (PPI) network of DEGs was constructed, and the hub genes were obtained using the STRING database and Cytoscape.
The ceRNA networks were constructed based on miRWalk and LncBase v2.
qRT-PCR was performed to confirm the expression of lncRNA MIR193BHG, PROX1-AS1, and GATA3-AS1.
ROC curves were performed to assess the clinical value of lncRNA MIR193BHG, PROX1-AS1, and GATA3-AS1 in the diagnosis of EOPE.
Results.
We found 6 hub genes (SPP1, CCR2, KIT, ENG, ACKR1, and FLT1) altered in placental tissues of EOPE and constructed a ceRNA network, including 21 lncRNAs, 3 mRNAs, and 69 miRNAs.
The expression of lncRNA MIR193BHG and GATA3-AS1 were elevated and showed good clinical values for diagnosing EOPE.
Conclusion.
This study provides novel insights into the lncRNA-related ceRNA network in EOPE and identified two lncRNAs as potential prognostic biomarkers in EOPE.
American Psychological Association (APA)
Zhang, Zhan& Wang, Ping& Zhang, Linlin& Huang, Chenxi& Gao, Junjun& Li, Yingying…[et al.]. 2020. Identification of Key Genes and Long Noncoding RNA-Associated Competing Endogenous RNA (ceRNA) Networks in Early-Onset Preeclampsia. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1131825
Modern Language Association (MLA)
Zhang, Zhan…[et al.]. Identification of Key Genes and Long Noncoding RNA-Associated Competing Endogenous RNA (ceRNA) Networks in Early-Onset Preeclampsia. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1131825
American Medical Association (AMA)
Zhang, Zhan& Wang, Ping& Zhang, Linlin& Huang, Chenxi& Gao, Junjun& Li, Yingying…[et al.]. Identification of Key Genes and Long Noncoding RNA-Associated Competing Endogenous RNA (ceRNA) Networks in Early-Onset Preeclampsia. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1131825
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131825