Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6
Joint Authors
Yang, Yonghong
Cho, William Chi-Shing
Zhang, Kai
Bi, Yanzhen
Wang, Quanyi
Wang, Quanquan
Zhang, Xiaobei
Shu, Zhenfeng
Li, Jiannan
Liu, Lili
Si, Chuanping
Hong, Feng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Gastric cancer is among the most common malignant tumors of the digestive tract.
Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer.
The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45 cells using microcarrier.
Sixty male C57BL/6 mice were randomly divided into three groups: a 2D group, an empty carrier group, and a 3D group, according to the coculture system of MKN45 and the microcarrier.
The mouse models were established by hypodermic injection.
Time to develop tumor, rate of tumor formation, and pathological features were observed in each group.
In the 3D group, the tumorigenesis time was short, while the rate of tumor formation was high (75%).
There was no detectable tumor formation in either the 2D or the empty carrier group.
Both H&E and immunohistochemical staining of the tumor xenograft showed characteristic evidence of human gastric neoplasms.
The present study successfully established a human gastric carcinoma model in immunocompetent mice, which provides a novel and valuable animal model for the cancer research and development of anticancer drugs.
American Psychological Association (APA)
Bi, Yanzhen& Wang, Quanyi& Yang, Yonghong& Wang, Quanquan& Zhang, Kai& Zhang, Xiaobei…[et al.]. 2020. Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132121
Modern Language Association (MLA)
Bi, Yanzhen…[et al.]. Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132121
American Medical Association (AMA)
Bi, Yanzhen& Wang, Quanyi& Yang, Yonghong& Wang, Quanquan& Zhang, Kai& Zhang, Xiaobei…[et al.]. Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132121
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132121