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miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma
Joint Authors
Chen, Kai
Li, Zhonghu
Zhang, Mengyun
Wang, Bo
Peng, Tao
Shen, Yanbing
Zhang, Jianxin
Ye, Jiaxin
Liu, Yu
Tang, Di
Peng, Minjie
Ma, Dandan
Xiao, Zhengkang
Zhang, Yujun
Jin, Weidong
Li, Xiaowu
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-06
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Background.
The asymptomatic onset, frequent recurrence, and poor prognosis of hepatocellular carcinoma (HCC) prompted us to identify new therapeutic targets or predictive markers of HCC diagnosis or prognosis.
Methods.
In this study, bioinformatics analysis was used to screen for target miRNAs from the open-access TCGA database.
Transwell assays, Western blotting, and qRT-PCR analyses were used to detect cellular functions and gene expression in HCC cells and samples.
A nude mouse tumorigenesis model was established to facilitate the observation of HCC progression.
Other assays included luciferase reporter assays, IHC, and survival analysis.
Results.
We found that the identified miR-876 from TCGA was expressed at low levels in HCC cell lines and that low miR-876 expression was corrected with liver cirrhosis, tumor thrombus, and TNM stage.
Further research revealed that miR-876 regulated cell invasion, EMT, and collagen expression by targeting POSTN expression.
miR-876 and POSTN were inversely correlated in HCC samples and associated with EMT status and liver fibrosis in clinical HCC tissues.
miR-876 inhibited the liver cancer progression in in vivo animal assays.
Finally, both miR-876 and POSTN were risk factors for HCC survival, and HCC patients with combined low miR-876 and high POSTN expression had worse prognosis.
Conclusions.
miR-876 inhibited HCC EMT and fibrosis by targeting POSTN, thus affecting HCC progression and prognosis.
miR-876 and POSTN may be useful therapeutic targets or prognostic markers of HCC.
American Psychological Association (APA)
Chen, Kai& Li, Zhonghu& Zhang, Mengyun& Wang, Bo& Peng, Tao& Shen, Yanbing…[et al.]. 2020. miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma. BioMed Research International،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1132190
Modern Language Association (MLA)
Chen, Kai…[et al.]. miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma. BioMed Research International No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1132190
American Medical Association (AMA)
Chen, Kai& Li, Zhonghu& Zhang, Mengyun& Wang, Bo& Peng, Tao& Shen, Yanbing…[et al.]. miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1132190
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132190