![](/images/graphics-bg.png)
Antigen Epitope Developed Based on Acinetobacter baumannii MacB Protein Can Provide Partial Immune Protection in Mice
Joint Authors
Song, Xiaojie
Zhao, Guanghui
Ding, Meiling
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Acinetobacter baumannii (A.
baumannii) is an important opportunistic pathogen widely present in medical environment.
Given its complex drug resistance, A.
baumannii poses a serious threat to the safety of critically ill patients.
Given the limited alternative antibiotics, nonantibiotic-based functional anti-A.
baumannii infection proteins must be developed.
In this study, we firstly used a series of biological software to predict potential epitopes in the MacB protein sequence and verified them by antibody recognition and lymphocyte proliferation tests.
We finally screened out B cell epitope 2, CD8+ T cell epitope 7, and CD4+ T cell epitope 11 and connected them to construct a recombinant antigen epitope (RAE).
The determination of IgG in the serum of immunised mice and cytokines in the supernatant of lymphocytes showed that the constructed epitope induced an immune response mediated by Th-1 cells.
Finally, the challenge experiment of A.
baumannii infection in mice confirmed that the epitope developed based on MacB, especially RAE, provided incomplete immune protection for mice.
American Psychological Association (APA)
Song, Xiaojie& Zhao, Guanghui& Ding, Meiling. 2020. Antigen Epitope Developed Based on Acinetobacter baumannii MacB Protein Can Provide Partial Immune Protection in Mice. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1132212
Modern Language Association (MLA)
Song, Xiaojie…[et al.]. Antigen Epitope Developed Based on Acinetobacter baumannii MacB Protein Can Provide Partial Immune Protection in Mice. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1132212
American Medical Association (AMA)
Song, Xiaojie& Zhao, Guanghui& Ding, Meiling. Antigen Epitope Developed Based on Acinetobacter baumannii MacB Protein Can Provide Partial Immune Protection in Mice. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1132212
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132212