Withania frutescens. L Extract: Phytochemical Characterization and Acute and Repeated Dose 28-Day Oral Toxicity Studies in Mice
Joint Authors
Mahmood, Hafiz Majid
Bourhia, Mohammed
Bari, Ahmed
Moussaoui, Abdelfattah EL
Jawhari, Fatima Zahra
Es-safi, Imane
Ali, Syed Saeed
Bousta, Dalila
Bari, Amina
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Withania frutescens.
L (W.
frutescens) is a perennial woody medicinal plant belonging to family Solanaceae largely used by the indigenous population to Morocco for the treatment of disease.
Objective.
The purpose of this study was to investigate the chemical composition, acute, and subacute toxicity of W.
frutescens extract in mice.
Materials and Methods.
The phytochemical composition of W.
frutescens extract was determined using a gas chromatograph (GC/MS).
Acute toxicity study was carried out in mice through oral administration of single doses 500 mg/kg, 1000 mg/kg, and 2000 mg/kg for 14 days.
Subacute toxicity was performed with oral administration of repeated doses 500 and 2000 mg/kg/day for 28 days.
Biochemical parameters (alanine aminotransferase, aspartate aminotransferase, urea, and creatinine), as well as histopathological changes potentially occurred in organs, (liver, kidney, and spleen) were evaluated.
Results.
The results of chromatographic analysis showed the richness of W.
frutescens extract in interesting phytochemical compounds majorly constituted of bicyclo[3.1.1]heptane, 6,6-dimethyl-2-methylene-(C10H16).
Regarding acute toxicity study, the results showed no clinical symptoms occurred in treated mice compared to the control group and no histological changes detected in analyzed organs of treated mice with dose put to 2000 mg/kg nor adverse effect on biochemical parameters.
Conclusion.
The outcome of this work showed no toxic effect of W.
frutescens in mice up to dose 2000 mg/kg bodyweight.
Therefore, this study could scientifically validate further traditional use with safety in the range of tested doses.
American Psychological Association (APA)
Moussaoui, Abdelfattah EL& Bourhia, Mohammed& Jawhari, Fatima Zahra& Es-safi, Imane& Ali, Syed Saeed& Bari, Ahmed…[et al.]. 2020. Withania frutescens. L Extract: Phytochemical Characterization and Acute and Repeated Dose 28-Day Oral Toxicity Studies in Mice. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132215
Modern Language Association (MLA)
Moussaoui, Abdelfattah EL…[et al.]. Withania frutescens. L Extract: Phytochemical Characterization and Acute and Repeated Dose 28-Day Oral Toxicity Studies in Mice. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132215
American Medical Association (AMA)
Moussaoui, Abdelfattah EL& Bourhia, Mohammed& Jawhari, Fatima Zahra& Es-safi, Imane& Ali, Syed Saeed& Bari, Ahmed…[et al.]. Withania frutescens. L Extract: Phytochemical Characterization and Acute and Repeated Dose 28-Day Oral Toxicity Studies in Mice. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132215
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132215