![](/images/graphics-bg.png)
Improvement of Ethanol Tolerance by Inactive Protoplast Fusion in Saccharomyces cerevisiae
Joint Authors
Yang, Jianming
Xin, Yi
Yang, Mei
Yin, Hua
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Saccharomyces cerevisiae is a typical fermentation yeast in beer production.
Improving ethanol tolerance of S.
cerevisiae will increase fermentation efficiency, thereby reducing capital costs.
Here, we found that S.
cerevisiae strain L exhibited a higher ethanol tolerance (14%, v/v) than the fermentative strain Q (10%, v/v).
In order to enhance the strain Q ethanol tolerance but preserve its fermentation property, protoplast fusion was performed with haploids from strain Q and L.
The fusant Q/L-f2 with 14% ethanol tolerance was obtained.
Meanwhile, the fermentation properties (flocculability, SO2 production, α-N assimilation rate, GSH production, etc.) of Q/L-f2 were similar to those of strain Q.
Therefore, our works established a series of high ethanol-tolerant strains in beer production.
Moreover, this demonstration of inactivated protoplast fusion in industrial S.
cerevisiae strain opens many doors for yeast-based biotechnological applications.
American Psychological Association (APA)
Xin, Yi& Yang, Mei& Yin, Hua& Yang, Jianming. 2020. Improvement of Ethanol Tolerance by Inactive Protoplast Fusion in Saccharomyces cerevisiae. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1132216
Modern Language Association (MLA)
Xin, Yi…[et al.]. Improvement of Ethanol Tolerance by Inactive Protoplast Fusion in Saccharomyces cerevisiae. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1132216
American Medical Association (AMA)
Xin, Yi& Yang, Mei& Yin, Hua& Yang, Jianming. Improvement of Ethanol Tolerance by Inactive Protoplast Fusion in Saccharomyces cerevisiae. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1132216
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132216