Polydeoxyribonucleotide Exerts Therapeutic Effect by Increasing VEGF and Inhibiting Inflammatory Cytokines in Ischemic Colitis Rats
Joint Authors
Kim, Chang-Ju
Kim, Sung-Eun
Ko, Il-Gyu
Jin, Jun-Jang
Hwang, Lakkyong
Kim, Sang-Hoon
Han, Jin-Hee
Jeon, Jung Won
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Ischemic colitis is resulted from an inadequate blood supply to a segment or entire colon.
Polydeoxyribonucleotide (PDRN), extracted from salmon sperm, has been reported to exert anti-inflammatory and anti-ischemic effects through the adenosine A2A receptor (A2AR).
We investigated whether PDRN possesses therapeutic effectiveness on ischemic colitis rats.
Ischemic colitis was induced by selective devascularization.
The skin temperature on the ischemic colitis-induced region was determined.
To assess the colonic damage score and collagen deposition, colonic tissue sections were stained with hematoxylin and eosin (H&E), and Masson trichrome staining was performed.
Western blot analysis for A2AR, vascular endothelial growth factor (VEGF), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6, Bax, Bcl-2, and extracellular signal-regulated kinase 1/2 (ERK1/2) was performed.
Skin temperature was increased and mucosal damage and collagen deposition were observed in the affected colonic tissues in the ischemic colitis rats.
Expressions of inflammatory cytokines (TNF-α, IL-1β, and IL-6) and inflammatory mediator (COX-2) were upregulated in the ischemic colitis rats.
Apoptosis was increased by decreasing the ratio of Bcl-2 to Bax and by suppressing the phosphorylated form of ERK1/2 expression in the ischemic colitis rats.
Treatment with PDRN alleviated mucosal damage reduced the expressions of inflammatory cytokines and COX-2 and inhibited apoptosis in the ischemic colitis rats.
PDRN treatment more enhanced the expressions of A2AR and VEGF in the ischemic colitis rats.
PDRN showed therapeutic effectiveness on ischemic colitis by increasing VEGF expression and inhibiting inflammatory cytokines and COX-2 through enhancing A2AR expression.
American Psychological Association (APA)
Kim, Sung-Eun& Ko, Il-Gyu& Jin, Jun-Jang& Hwang, Lakkyong& Kim, Chang-Ju& Kim, Sang-Hoon…[et al.]. 2020. Polydeoxyribonucleotide Exerts Therapeutic Effect by Increasing VEGF and Inhibiting Inflammatory Cytokines in Ischemic Colitis Rats. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1132385
Modern Language Association (MLA)
Kim, Sung-Eun…[et al.]. Polydeoxyribonucleotide Exerts Therapeutic Effect by Increasing VEGF and Inhibiting Inflammatory Cytokines in Ischemic Colitis Rats. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1132385
American Medical Association (AMA)
Kim, Sung-Eun& Ko, Il-Gyu& Jin, Jun-Jang& Hwang, Lakkyong& Kim, Chang-Ju& Kim, Sang-Hoon…[et al.]. Polydeoxyribonucleotide Exerts Therapeutic Effect by Increasing VEGF and Inhibiting Inflammatory Cytokines in Ischemic Colitis Rats. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1132385
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132385