Effects of High-Fat Diet and Exercise Intervention on the Metabolism Regulation of Infant Mice

Abstract EN

Maternal exercise is crucial for promoting the health of the offspring.

Previous studies showed that long-term maternal exercise improves energy metabolism during pregnancy.

Whether swimming exercise can reverse the metabolic disorders caused by high-fat exposure in the early life of the offspring is yet to be elucidated.

Three-week-old C57BL/6 female mice were randomly assigned to the standard chow diet group (SC), standard chow diet and exercise group (SC-Ex), high-fat diet group (HFD), and high-fat diet and exercise group (HFD-Ex).

After swimming intervention for 13 weeks, male and female mice were caged, and the exercise intervention lasted until delivery.

Then, the mothers were fed standard chow diet.

A total of 8 offsprings/group were randomly selected after 4 weeks of lactation for GTT and ITT.

After body composition analysis, the mice were sacrificed to obtain specimens.

The levels of metabolism factors and IL-6 were measured by suspension microarray.

Subsequently, 15 min after starting the GTT and ITT, the curve detected significant difference between the HFD and other groups.

The body fat percentage of the HFD-Ex offspring was significantly lower than that of HFD offspring (p<0.05) irrespective of the gender.

The levels of IL-6 and TG in the male offspring in the HFD-Ex group were improved significantly (p<0.05).

Compared to the HFD offspring, serum glucose and GIP in the female offspring in the HFD-Ex group was significantly reduced (p<0.05).

Long-term exercise of the mother effectively improved the metabolic disorder caused by high-fat exposure in the infant offspring.

Thus, the metabolic inheritance of the offspring is gender-dependent; the maternal metabolism can make male offspring genetically susceptible.