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Developmental and Tissue Patterns of the Basal Expression of Chicken Avian β-Defensins
Joint Authors
Gong, Yujie
Wen, Xueting
Lyu, Wentao
Xiao, Yingping
Yang, Hua
Zhang, Long
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Defensins are a class of antimicrobial peptides in vertebrates that function as the first line of innate immunity with potent antimicrobial and immunomodulatory activities.
Fourteen defensins, namely, avian β-defensin 1 to 14 (AvBD1-14), have been identified in chickens.
Before characterizing the role of AvBDs in innate immunity during the early development of chickens, we collected tissue segments from the liver, spleen, and gastrointestinal (GI) tract including the esophagus, crop, proventriculus, gizzard, duodenum, jejunum, ileum, cecum, and colon from broilers at days 1, 3, 7, 14, and 28.
After RNA isolation and reverse transcription, we determined the expression levels of the 14 AvBD genes in these tissues during the first 28 days after hatching by real-time PCR.
The results suggested the AvBDs were widely expressed in the chicken liver, spleen, and gastrointestinal (GI) tract.
Interestingly, we did not detect AvBD11 expressed in the GI tract, even in the liver and spleen.
Additionally, AvBDs were differentially expressed in the chicken GI tract.
AvBD5 and AvBD14 were expressed most abundantly in the proximal GI tract, especially the esophagus and crop.
Moreover, AvBD5, AvBD7, AvBD9, and AvBD14 were expressed in an inverted-V pattern with the peak being the observed expression at days 3, 7, or 14 in the chicken spleen, esophagus, duodenum, and cecum.
Other AvBDs presented biphasic or inverted-V expression patterns in different tissues.
The expression levels of all detected AvBDs were strengthened after hatching rather than decreasing steadily.
Therefore, AvBDs were found to be expressed widely in the chicken liver, spleen, and GI tract and their expression levels were primarily up regulated during the early development of chicken, implying the potential essential roles of AvBDs in early innate defense and infection resistance of chickens.
American Psychological Association (APA)
Lyu, Wentao& Zhang, Long& Gong, Yujie& Wen, Xueting& Xiao, Yingping& Yang, Hua. 2020. Developmental and Tissue Patterns of the Basal Expression of Chicken Avian β-Defensins. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1132595
Modern Language Association (MLA)
Lyu, Wentao…[et al.]. Developmental and Tissue Patterns of the Basal Expression of Chicken Avian β-Defensins. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1132595
American Medical Association (AMA)
Lyu, Wentao& Zhang, Long& Gong, Yujie& Wen, Xueting& Xiao, Yingping& Yang, Hua. Developmental and Tissue Patterns of the Basal Expression of Chicken Avian β-Defensins. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1132595
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132595