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Skin Pigmentation Differences between Mongolian, Korean, and Uzbekistan Ancient Human DNA Samples
Joint Authors
Kim, Kyungyong
Bazarragchaa, Munkhtsetseg
Uuganbayar, Udval
Lee, Kwang-Ho
Kim, Kijeong
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
This study reports the use of real-time PCR to identify the SNP rs1545397 in the intron region on the OCA2 gene from ancient and degraded DNA isolated from ancient human bones from Mongolia, Korea, and Uzbekistan.
This SNP is a marker for skin pigmentation.
LightCycler-based probes (HybProbes) were designed.
A LightCycler (version 2.0) system was used for the real-time PCR.
Results.
The results of the real-time PCRs of three different genotypes of SNP rs1545397 were compared with those of the direct sequencing.
Melting curve analysis was used for genotype determination.
Three genotypes were distinguished: the homozygous T (T/T) SNP type formed a distinct melting peak at 53.3±0.14°C, the homozygous A (A/A) SNP type formed a distinct melting peak at 57.8±0.12°C, and the heterozygous A/T SNP type formed two distinct melting peaks at 53.3±0.17°C and 57.8±0.15°C.
Mongolian aDNA samples tested in this study carried all three types of the SNP (A/T, A/A, and T/T) with no distinctly predominant type observed.
In contrast, Korean aDNA samples carried the Asian genotype (T/T), while the Uzbekistan aDNA samples carried the European genotype (A/A) more often than the Asian genotype (T/T).
Conclusions.
Human Mongolian aDNA samples had A/T, A/A, and T/T SNP rs1545397 with no distinct predominant genotype.
When combined with the archeological and aDNA studies of other coupling morphologies with aDNA, our results infer that Mongolia’s prehistoric population had considerable heterogeneity of skin color and morphological traits and that in the Neolithic period, a Eurasian or mixed population inhabited the western part of Mongolia.
American Psychological Association (APA)
Bazarragchaa, Munkhtsetseg& Uuganbayar, Udval& Lee, Kwang-Ho& Kim, Kyungyong& Kim, Kijeong. 2020. Skin Pigmentation Differences between Mongolian, Korean, and Uzbekistan Ancient Human DNA Samples. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132608
Modern Language Association (MLA)
Bazarragchaa, Munkhtsetseg…[et al.]. Skin Pigmentation Differences between Mongolian, Korean, and Uzbekistan Ancient Human DNA Samples. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132608
American Medical Association (AMA)
Bazarragchaa, Munkhtsetseg& Uuganbayar, Udval& Lee, Kwang-Ho& Kim, Kyungyong& Kim, Kijeong. Skin Pigmentation Differences between Mongolian, Korean, and Uzbekistan Ancient Human DNA Samples. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132608
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132608