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GDF15 Repression Contributes to 5-Fluorouracil Resistance in Human Colon Cancer by Regulating Epithelial-Mesenchymal Transition and Apoptosis
Joint Authors
Ma, Xiao
Ma, Nina
Zheng, Xixi
Hu, Jiexuan
Cao, Bangwei
Li, Xiao
Wang, Bin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Chemotherapy based on 5-fluorouracil (5-FU) is the standard approach for colon cancer treatment, and resistance to 5-FU is a significant obstacle in the clinical treatment of colon cancer.
However, the mechanisms underlying 5-FU resistance in colon cancer cells remain largely unknown.
This study aimed at determining whether 5-FU-resistant colon cancer cells undergo epithelial-mesenchymal transition (EMT) and apoptosis and the role of GDF15—a member of the transforming growth factor β/bone morphogenetic protein super family and a protein known to be involved in cancer progression—in the regulation of EMT and apoptosis of these cells, along with the underlying mechanisms.
In vitro apoptosis detection assay, growth inhibition assay, transwell, and wound healing experiments revealed that 5-FU-resistant colon cancer cells possessed enhanced EMT and antiapoptotic ability.
These cells also showed a stronger tendency to proliferate and metastasize in vivo.
Quantitative reverse transcription-PCR and western blotting revealed that 5-FU-resistant colon cancer cells expressed lower levels of growth differentiation factor 15 (GDF15) than did 5-FU-sensitive colon cancer cells.
Moreover, the transient GDF15 overexpression resensitized 5-FU-resistant colon cells to 5-FU.
Collectively, these findings indicate the mechanism underlying the 5-FU resistance of colon cancer cells and provide new therapeutic targets for improving the prognosis of colon cancer patients.
American Psychological Association (APA)
Wang, Bin& Ma, Nina& Zheng, Xixi& Li, Xiao& Ma, Xiao& Hu, Jiexuan…[et al.]. 2020. GDF15 Repression Contributes to 5-Fluorouracil Resistance in Human Colon Cancer by Regulating Epithelial-Mesenchymal Transition and Apoptosis. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1132744
Modern Language Association (MLA)
Wang, Bin…[et al.]. GDF15 Repression Contributes to 5-Fluorouracil Resistance in Human Colon Cancer by Regulating Epithelial-Mesenchymal Transition and Apoptosis. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1132744
American Medical Association (AMA)
Wang, Bin& Ma, Nina& Zheng, Xixi& Li, Xiao& Ma, Xiao& Hu, Jiexuan…[et al.]. GDF15 Repression Contributes to 5-Fluorouracil Resistance in Human Colon Cancer by Regulating Epithelial-Mesenchymal Transition and Apoptosis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1132744
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132744