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Protective Effects of Crocetin against Radiation-Induced Injury in Intestinal Epithelial Cells
Joint Authors
Zhang, Chen
Chen, Kequan
Wang, Jinghua
Zheng, Zhongwen
Luo, Yujun
Zhou, Weijie
Zhuo, Zewei
Liang, Jun
Sha, Weihong
Chen, Hao
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background and Aims.
Treatment options for radiation-induced intestinal injury (RIII) are limited.
Crocetin has been demonstrated to exert antioxidant, antiapoptotic, and anti-inflammatory effects on various diseases.
Here, we investigate the effects of crocetin on RIII in vitro.
Materials and Method.
IEC-6 cells exposed to 10 Gy of radiation were treated with different doses of crocetin (0, 0.1, 1, 10, and 100 μM), and cell viability was assessed by CCK-8.
The levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), malondialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interferon-γ (IFN-γ) in culture supernatants were measured using colorimetric and ELISA kits, respectively.
Cellular apoptosis was evaluated by Annexin V/PI double staining.
Results.
Crocetin dose-dependently improved the survival of irradiated IEC-6 cells with the optimal dose of 10 μM, as indicated by the reduction of cellular apoptosis, decreased levels of MDA, MPO, and proinflammatory cytokines (TNF-α, IL-1β, and IFN-γ), and increased activities of antioxidative enzymes (SOD, CAT, and GPx).
Conclusion.
Our findings demonstrated that crocetin alleviated radiation-induced injury in intestinal epithelial cells, offering a promising agent for radioprotection.
American Psychological Association (APA)
Zhang, Chen& Chen, Kequan& Wang, Jinghua& Zheng, Zhongwen& Luo, Yujun& Zhou, Weijie…[et al.]. 2020. Protective Effects of Crocetin against Radiation-Induced Injury in Intestinal Epithelial Cells. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1132798
Modern Language Association (MLA)
Zhang, Chen…[et al.]. Protective Effects of Crocetin against Radiation-Induced Injury in Intestinal Epithelial Cells. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1132798
American Medical Association (AMA)
Zhang, Chen& Chen, Kequan& Wang, Jinghua& Zheng, Zhongwen& Luo, Yujun& Zhou, Weijie…[et al.]. Protective Effects of Crocetin against Radiation-Induced Injury in Intestinal Epithelial Cells. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1132798
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132798