IL-4 Alleviates Ischaemia-Reperfusion Injury by Inducing Kupffer Cells M2 Polarization via STAT6-JMJD3 Pathway after Rat Liver Transplantation
Joint Authors
Li, Jin-Zheng
Wu, Hao
Wu, Yakun
Deng, Minghua
Wang, Jingyuan
Wang, Menghao
Cao, Ding
Gong, Jianping
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Liver ischaemia-reperfusion injury (IRI) remains a problem in liver transplantation.
Interleukin-4 (IL-4) has been found to reduce liver IRI, but the exact mechanism remains unclear.
Methods.
Donor livers were infused with recombinant IL-4 or normal saline during cold storage, and the hepatocellular apoptosis and the inflammatory response were detected.
The effect of IL-4 treatment on Kupffer cells (KCs) polarization and expression of the STAT6-JMJD3 pathway was evaluated in vivo and in vitro.
KCs in donor livers were depleted by clodronate liposome treatment or JMJD3 was inhibited by GSK-J4 before liver transplantation to determine whether the protective effect of IL-4 treatment was dependent on KCs.
Results.
IL-4 treatment decreased sALT and sAST levels and alleviated hepatocellular apoptosis and inflammation at 6 h after liver transplantation.
IL-4 treatment induced KCs alternatively activated (M2) polarization in vitro and in vivo, and the expression of STAT6 and JMJD3 was increased.
JMJD3 knockdown abolished KCs M2 polarization and reduced the antiapoptotic and anti-inflammatory effects induced by IL-4 treatment in vitro.
In addition, the protection of IL-4 treatment against IRI induced by liver transplantation was significantly reduced after the depletion of KCs or the inhibition of JMJD3 in donor livers.
Conclusions.
IL-4 treatment-induced KCs M2 polarization was dependent on the STAT6-JMJD3 pathway and protected liver grafts from IRI after liver transplantation.
American Psychological Association (APA)
Deng, Minghua& Wang, Jingyuan& Wu, Hao& Wang, Menghao& Cao, Ding& Li, Jin-Zheng…[et al.]. 2020. IL-4 Alleviates Ischaemia-Reperfusion Injury by Inducing Kupffer Cells M2 Polarization via STAT6-JMJD3 Pathway after Rat Liver Transplantation. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1132855
Modern Language Association (MLA)
Deng, Minghua…[et al.]. IL-4 Alleviates Ischaemia-Reperfusion Injury by Inducing Kupffer Cells M2 Polarization via STAT6-JMJD3 Pathway after Rat Liver Transplantation. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1132855
American Medical Association (AMA)
Deng, Minghua& Wang, Jingyuan& Wu, Hao& Wang, Menghao& Cao, Ding& Li, Jin-Zheng…[et al.]. IL-4 Alleviates Ischaemia-Reperfusion Injury by Inducing Kupffer Cells M2 Polarization via STAT6-JMJD3 Pathway after Rat Liver Transplantation. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1132855
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132855