Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression
Joint Authors
Zhang, Tie-Ning
Liu, Chun-Feng
Zhu, Xiao Geng
Wen, Ri
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sepsis-induced myocardial depression has high mortality and is very common in intensive care units.
Previous studies have found that microRNAs play an important role in regulating sepsis-induced myocardial depression.
miR-150-5p is involved in many biological processes; however, the mechanism underlying its role in sepsis-induced myocardial depression is still unclear.
In this study, we generated rat models of septic shock induced by lipopolysaccharide.
Whole genomic RNA sequencing was performed on 12 left ventricles collected after LPS treatment to identify miRNAs.
Most of the target genes of the differently expressed microRNAs were involved in apoptosis, according to Gene Ontology.
We also observed apoptosis in the heart tissue and in H9C2 cardiomyocytes stimulated with lipopolysaccharide, indicating that cell apoptosis may be an important mechanism in sepsis-induced myocardial depression.
Furthermore, the expression of miR-150-5p was reduced, and overexpression of miR-150-5p with mimics resulted in a decrease in apoptosis, decreased expression of cleaved caspase3 and Bax, and increased expression of Bcl-2.
Additionally, after H9C2 cells were transfected with miR-150-5p mimics or an inhibitor, the expression of Akt2 decreased or increased, respectively.
These findings suggest that miR-150-5p can alleviate apoptosis and may be a novel therapeutic target for sepsis-induced myocardial depression.
American Psychological Association (APA)
Zhu, Xiao Geng& Zhang, Tie-Ning& Wen, Ri& Liu, Chun-Feng. 2020. Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1132893
Modern Language Association (MLA)
Zhu, Xiao Geng…[et al.]. Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1132893
American Medical Association (AMA)
Zhu, Xiao Geng& Zhang, Tie-Ning& Wen, Ri& Liu, Chun-Feng. Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1132893
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132893