Mechanism of Antibacterial Activity of Bacillus amyloliquefaciens C-1 Lipopeptide toward Anaerobic Clostridium difficile
Joint Authors
Lv, Jia
Da, Rong
Cheng, Yue
Tuo, Xiaohong
Wei, Jie
Jiang, Kaichong
Monisayo, Adediji Omolade
Han, Bei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Probiotics may offer an attractive alternative for standard antibiotic therapy to treat Clostridium difficile infections (CDI).
In this study, the antibacterial mechanism in vitro of newly isolated B.
amyloliquefaciens C-1 against C.
difficile was investigated.
The lipopeptides surfactin, iturin, and fengycin produced by C-1 strongly inhibited C.
difficile growth and viability.
Systematic research of the bacteriostatic mechanism showed that the C-1 lipopeptides damage the integrity of the C.
difficile cell wall and cell membrane.
In addition, the lipopeptide binds to C.
difficile genomic DNA, leading to cell death.
Genome resequencing revealed many important antimicrobial compound-encoding clusters, including six nonribosomal peptides (surfactins (srfABCD), iturins (ituABCD), fengycins (fenABCDE), bacillibactin (bmyABC), teichuronic, and bacilysin) and three polyketides (bacillaene (baeEDLMNJRS), difficidin (difABCDEFGHIJ), and macrolactin (mlnABCDEFGHI)).
In addition, there were other beneficial genes, such as phospholipase and seven siderophore biosynthesis gene clusters, which may contribute synergistically to the antibacterial activity of B.
amyloliquefaciens C-1.
We suggest that proper application of antimicrobial peptides may be effective in C.
difficile control.
American Psychological Association (APA)
Lv, Jia& Da, Rong& Cheng, Yue& Tuo, Xiaohong& Wei, Jie& Jiang, Kaichong…[et al.]. 2020. Mechanism of Antibacterial Activity of Bacillus amyloliquefaciens C-1 Lipopeptide toward Anaerobic Clostridium difficile. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1133003
Modern Language Association (MLA)
Lv, Jia…[et al.]. Mechanism of Antibacterial Activity of Bacillus amyloliquefaciens C-1 Lipopeptide toward Anaerobic Clostridium difficile. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1133003
American Medical Association (AMA)
Lv, Jia& Da, Rong& Cheng, Yue& Tuo, Xiaohong& Wei, Jie& Jiang, Kaichong…[et al.]. Mechanism of Antibacterial Activity of Bacillus amyloliquefaciens C-1 Lipopeptide toward Anaerobic Clostridium difficile. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1133003
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133003