Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway
Joint Authors
Yang, Sheng-Li
Xiong, Zhi-Fan
He, Xiaoxiao
Huang, Zaozao
Liu, Ping
Li, Qiuting
Wang, Mengmeng
Qiu, Mengjun
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
We aimed to investigate whether apatinib has an inhibitory effect on the invasion and metastasis of liver cancer in vitro.
Methods.
The anti-invasion and antimetastasis effects of apatinib in HepG2, Hep3B,Huh7 and SMMC-7721 liver cancer cell lines were tested by the wound-healing and transwell invasion assays.
Real-time PCR and Western blot were used to detect the influence of apatinib on the gene expression of MMPs, TIMPs, and constituents of the NF-κB signaling pathway in Hep3B and HepG2 liver cell lines.
Results.
Apatinib has a significant inhibitory effect on the metastasis and invasion of liver cancer cells.
The expression levels of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-10, MMP-11, and MMP-16 were downregulated, while the expression levels of TIMP-3 and TIMP-4 were upregulated by apatinib treatment at both the mRNA and protein levels.
The phosphorylation of IκBα and NF-κB p65 was significantly reduced compared with that in the control group.
Conclusions.
Apatinib inhibits the invasion and metastasis of human liver cancer cells by downregulating the expression of MMP-related genes.
This may be achieved by inhibiting the activation of the NF-κB signaling pathway.
American Psychological Association (APA)
He, Xiaoxiao& Huang, Zaozao& Liu, Ping& Li, Qiuting& Wang, Mengmeng& Qiu, Mengjun…[et al.]. 2020. Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133014
Modern Language Association (MLA)
He, Xiaoxiao…[et al.]. Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1133014
American Medical Association (AMA)
He, Xiaoxiao& Huang, Zaozao& Liu, Ping& Li, Qiuting& Wang, Mengmeng& Qiu, Mengjun…[et al.]. Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133014