Differential Expression of Urinary Exosomal Small RNAs in Idiopathic Membranous Nephropathy
Joint Authors
Zhang, Jinshi
Zhu, Yifan
Cai, Ruyi
Jin, Juan
He, Qiang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Idiopathic membranous nephropathy (IMN) is a major cause of adult nephrotic syndromes, and reliable noninvasive biomarkers for diagnosis and monitoring are urgently needed.
In this study, we performed small RNA (sRNA) sequencing to explore sRNA profiles of urinary exosomes derived from IMN patients and healthy controls (CON) to provide clues for identifying novel noninvasive sRNA biomarkers for IMN.
Methods.
Urine samples were collected from five healthy controls and six patients with IMN.
High-throughput sequencing was used to screen sRNA expression profiles of urinary exosomes from patients with IMN in two independent cohorts.
Results.
Urinary exosomes were successfully isolated and used to obtain exosomal sRNAs.
We screened 131 differentially expressed miRNAs, including 28 specifically expressed miRNAs, then explored the top 10 specifically expressed miRNAs in all IMN individuals.
The specifically expressed miRNAs and differentially expressed miRNAs provide potential biomarkers for IMN.
Additionally, we discovered numerous sRNAs derived from genomic repetitive sequences, which could represent an exciting new area of research.
Conclusion.
Herein, we revealed significant differences in expression profiles of urinary exosomal miRNAs and repetitive region-derived sRNAs between patients with IMN and healthy controls.
The findings could facilitate the development of potential molecular targets for membranous nephropathy.
American Psychological Association (APA)
Zhang, Jinshi& Zhu, Yifan& Cai, Ruyi& Jin, Juan& He, Qiang. 2020. Differential Expression of Urinary Exosomal Small RNAs in Idiopathic Membranous Nephropathy. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1133042
Modern Language Association (MLA)
Zhang, Jinshi…[et al.]. Differential Expression of Urinary Exosomal Small RNAs in Idiopathic Membranous Nephropathy. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1133042
American Medical Association (AMA)
Zhang, Jinshi& Zhu, Yifan& Cai, Ruyi& Jin, Juan& He, Qiang. Differential Expression of Urinary Exosomal Small RNAs in Idiopathic Membranous Nephropathy. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1133042
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133042