Downregulated miR-204 Promotes Skeletal Muscle Regeneration
Joint Authors
Tan, Ya
Shen, Linyuan
Gan, Mailin
Fan, Yuan
Cheng, Xiao
Zheng, Ting
Niu, Lili
Chen, Lei
Jiang, Dongmei
Li, Xuewei
Zhang, Shunhua
Zhu, Li
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Skeletal muscle is the most abundant and a highly plastic tissue of the mammals, especially when it comes to regenerate after trauma, but there is limited information about the mechanism of muscle repair and its regeneration.
In the present study, we found that miR-204 is downregulated after skeletal muscle injury.
In vitro experiments showed that over-expression of miR-204 by transfecting with miR-204 mimics suppressed C2C12 cell proliferation, migration, and blocked subsequent differentiation, whereas inhibition of miR-204 by transfecting with miR-204 inhibitor showed the converse effects.
Furthermore, through the dual luciferase reporter system, we demonstrated that miR-204 can target the 3’UTR regions of Pax7, IGF1, and Mef2c and inhibit their expression.
Taken together, our results suggest that Pax7, IGF1, and Mef2c are the target genes of miR-204 in the process of myoblasts proliferation, cell migration, and differentiation, respectively, and may contribute to mouse skeletal muscle regeneration.
Our results may provide new ideas and references for the skeletal muscle study and may also provide therapeutic strategies of skeletal muscle injury.
American Psychological Association (APA)
Tan, Ya& Shen, Linyuan& Gan, Mailin& Fan, Yuan& Cheng, Xiao& Zheng, Ting…[et al.]. 2020. Downregulated miR-204 Promotes Skeletal Muscle Regeneration. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133050
Modern Language Association (MLA)
Tan, Ya…[et al.]. Downregulated miR-204 Promotes Skeletal Muscle Regeneration. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1133050
American Medical Association (AMA)
Tan, Ya& Shen, Linyuan& Gan, Mailin& Fan, Yuan& Cheng, Xiao& Zheng, Ting…[et al.]. Downregulated miR-204 Promotes Skeletal Muscle Regeneration. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133050
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133050