Characteristic and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Medicinal Plants of Schisandraceae
Joint Authors
Li, Shengnan
Zhang, Tingting
Ao, Wuliji
Zhang, Dachuan
Wang, Jiahao
Xu, Liang
Xing, Yanping
Yang, Yanyun
Bao, Guihua
Kang, Tingguo
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Schisandra chinensis, which has a high development value, has long been used as medicine.
Its mature fruits (called Wuweizi in Chinese) have long been used in the famous traditional Chinese medicine (TCM) recorded in the “Chinese Pharmacopoeia.” Chloroplasts (CP) are the highly conserved primitive organelles in plants, which can serve as the foundation for plant classification and identification.
This study introduced the structures of the CP genomes of three Schisandraceae species and analyzed their phylogenetic relationships.
Comparative analyses on the three complete chloroplast genomes can provide us with useful knowledge to identify the three plants.
In this study, approximately 5 g fresh leaves were harvested for chloroplast DNA isolation according to the improved extraction method.
A total of three chloroplast DNAs were extracted.
Afterwards, the chloroplast genomes were reconstructed using denovo combined with reference-guided assemblies.
General characteristics of the chloroplast genome and genome comparison with three Schisandraceae species was analyzed by corresponding software.
The total sizes of complete chloroplast genomes of S.
chinensis, S.
sphenanthera, and Kadsura coccinea were 146875 bp, 146842 bp, and 145399 bp, respectively.
Altogether, 124 genes were annotated, including 82 protein-coding genes, 34 tRNAs, and 8 rRNAs of all 3 species.
In SSR analysis, only S.
chinensis was annotated to hexanucleotides.
Moreover, comparative analysis of chloroplast Schisandraceae genome sequences revealed that the gene order and gene content were slightly different among Schisandraceae species.
Finally, phylogenetic trees were reconstructed, based on the genome-wide SNPs of 38 species.
The method can be used to identify and differentially analyze Schisandraceae plants and offer useful information for phylogenetics as well as further studies on traditional medicinal plants.
American Psychological Association (APA)
Zhang, Dachuan& Wang, Jiahao& Xu, Liang& Xing, Yanping& Zhang, Tingting& Li, Shengnan…[et al.]. 2020. Characteristic and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Medicinal Plants of Schisandraceae. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1133277
Modern Language Association (MLA)
Zhang, Dachuan…[et al.]. Characteristic and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Medicinal Plants of Schisandraceae. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1133277
American Medical Association (AMA)
Zhang, Dachuan& Wang, Jiahao& Xu, Liang& Xing, Yanping& Zhang, Tingting& Li, Shengnan…[et al.]. Characteristic and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Medicinal Plants of Schisandraceae. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1133277
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133277