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Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY
Joint Authors
Sengul, Gulgun
Liang, Huazheng
Furlong, Teri M.
Paxinos, George
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The organization of the mouse spinal dorsal horn has been delineated in 2D for the six Rexed laminae in our publication Atlas of the Spinal Cord: Mouse, Rat, Rhesus, Marmoset, and Human.
In the present study, the tissue clearing technique CLARITY was used to observe the cyto- and chemoarchitecture of the mouse spinal cord in 3D, using a variety of immunohistochemical markers.
We confirm prior observations regarding the location of glycine and serotonin immunoreactivities.
Novel observations include the demonstration of numerous calcitonin gene-related peptide (CGRP) perikarya, as well as CGRP fibers and terminals in all laminae of the dorsal horn.
We also observed sparse choline acetyltransferase (ChAT) immunoreactivity in small perikarya and fibers and terminals in all dorsal horn laminae, while gamma aminobutyric acid (GABA) and glutamate decarboxylase-67 (GAD67) immunoreactivities were found only in small perikarya and fibers.
Finally, numerous serotonergic fibers were observed in all laminae of the dorsal horn.
In conclusion, CLARITY confirmed the 2D immunohistochemical properties of the spinal cord.
Furthermore, we observed novel anatomical characteristics of the spinal cord and demonstrated that CLARITY can be used on spinal cord tissue to examine many proteins of interest.
American Psychological Association (APA)
Sengul, Gulgun& Liang, Huazheng& Furlong, Teri M.& Paxinos, George. 2020. Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1133384
Modern Language Association (MLA)
Sengul, Gulgun…[et al.]. Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1133384
American Medical Association (AMA)
Sengul, Gulgun& Liang, Huazheng& Furlong, Teri M.& Paxinos, George. Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1133384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133384