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Cucurbita ficifolia Fruit Extract Induces Tp53Caspase-Mediated Apoptosis in MCF-7 Breast Cancer Cells
Joint Authors
Alshammari, Ghedeir M.
Balakrishnan, Aristatile
Alshatwi, Ali A.
Al-Khalifa, Abdulrahman
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The second most biggest cancer worldwide is breast cancer.
There is an increasing need for safer, effective, and affordable drug candidates from natural sources to treat breast cancer.
In the present investigation, the anticancer effect of Cucurbita ficifolia Bouché (C.
ficifolia) fruit extract was tested on the human breast cancer cells such as MCF-7.
The cells were exposed with different doses of C.
ficifolia, for the assessment of IC50 concentrations on the MCF-7 cell lines for 24 hs.
The effect of C.
ficifolia fruit extract on morphological and apoptotic changes were evaluated by specific fluorescence staining techniques and real-time PCR in a time-dependent manner for 24 hs and 48 hs.
The IC50 value for C.
ficifolia fruit extract was found to be 90 μg/mL.
Morphological alteration and apoptotic distinctiveness aspect like chromatin condensation and nuclear fragmentation were noticed in C.
ficifolia extract exposed breast cancer cells.
Further, we observed that C.
ficifolia extract-induced programmed cell death in the MCF-7 cells were mediated with the elevated expression of the tumor suppressor gene such as p53 and apoptotic markers such as caspase-8, caspase-9, caspase-3, fatty acid synthase (FAS), Fas-associated protein with death domain (FADD), Bcl-2 homologous antagonist/killer (BAK), and Bcl-2-associated X protein (BAX).
These observations established that C.
ficifolia significantly concealed the cell division and provoked p53/caspase-mediated programmed cell death.
Further, we noticed that this cell death in MCF-7 cells is concentration and time dependent.
As evaluated through the comet assay, C.
ficifolia induced DNA damage; further upon increasing the duration of the treatment, the DNA damage was higher than before.
Thus, our study concludes that C.
ficifolia could serve as an effective anticancer agent through vital gene modulation.
American Psychological Association (APA)
Alshammari, Ghedeir M.& Balakrishnan, Aristatile& Alshatwi, Ali A.& Al-Khalifa, Abdulrahman. 2020. Cucurbita ficifolia Fruit Extract Induces Tp53Caspase-Mediated Apoptosis in MCF-7 Breast Cancer Cells. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1133400
Modern Language Association (MLA)
Alshammari, Ghedeir M.…[et al.]. Cucurbita ficifolia Fruit Extract Induces Tp53Caspase-Mediated Apoptosis in MCF-7 Breast Cancer Cells. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1133400
American Medical Association (AMA)
Alshammari, Ghedeir M.& Balakrishnan, Aristatile& Alshatwi, Ali A.& Al-Khalifa, Abdulrahman. Cucurbita ficifolia Fruit Extract Induces Tp53Caspase-Mediated Apoptosis in MCF-7 Breast Cancer Cells. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1133400
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133400