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Hepatoprotective Activity of Leptadenia hastata (Asclepiadaceae) on Acetaminophen-Induced Toxicity in Mice: In Vivo Study and Characterization of Bioactive Compounds through Molecular Docking Approaches
Joint Authors
Galani, Borris R. T.
Owona, Brice A.
Chuisseu, Dieudonné P. D.
Machewere, Esaïe
Ngantchouko, Claude B. N.
Moundipa, Paul F.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-01
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background and Objectives.
Leptadenia hastata is a liana from the family of Asclepiadaceae used in tropical Africa to treat diabetes mellitus.
In this study, we investigated its hepatoprotective mechanisms on acetaminophen- (APAP-) induced toxicity through in vivo and in silico approaches.
Materials and Methods.
Various aqueous extracts were prepared from this plant and preadministered per os to albino mice 3 h before APAP administration, once daily for one week.
Animals from the normal group were given only distilled water while those from negative control received only APAP 250 mg/kg.
After treatment, mice were sacrificed, the liver was collected for histopathology analysis, and different biochemical markers (alanine aminotransferase (ALT), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor-alpha (TNFα)) were measured.
The content of the active extract was analyzed by HPLC/UV.
Molecular docking was conducted using iGEMDOCK software, and the drug-likeness and pharmacokinetic profiles were evaluated using Swiss ADME.
Results.
APAP administration significantly increased (p<0.001) ALT in liver homogenates when compared to normal controls whereas the stem decoction at 250 mg/kg significantly (p<0.001) reduced this activity to a normal value comparable to silymarin 50 mg/kg which is better than leaf and root extracts.
Moreover, the stem decoction also significantly reduced the MDA levels (p<0.05) and increased those of GSH, SOD, and CAT (p<0.001) at doses of 250 and 500 mg/kg compared to the negative control.
A significant (p<0.001) decrease of TNFα levels and leukocyte infiltration was observed following treatment with this extract.
The HPLC/UV analysis of the decoction revealed the presence of dihydroxycoumarin, quinine, and scopoletin with the following retention times: 2.6, 5.1, and 7.01 min, respectively.
In silico studies showed that quinine and dihydroxycoumarin had great potentials to be orally administered drugs and possessed strong binding affinities with TNFα, TNF receptor, cyclooxygenase-2, iNOS, cytochrome P450 2E1, and GSH reductase.
Conclusion.
Based on these results, L.
hastata could be considered a source of promising hepatoprotective compounds with antioxidant and anti-inflammatory properties.
American Psychological Association (APA)
Galani, Borris R. T.& Owona, Brice A.& Chuisseu, Dieudonné P. D.& Machewere, Esaïe& Ngantchouko, Claude B. N.& Moundipa, Paul F.. 2020. Hepatoprotective Activity of Leptadenia hastata (Asclepiadaceae) on Acetaminophen-Induced Toxicity in Mice: In Vivo Study and Characterization of Bioactive Compounds through Molecular Docking Approaches. BioMed Research International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1133434
Modern Language Association (MLA)
Galani, Borris R. T.…[et al.]. Hepatoprotective Activity of Leptadenia hastata (Asclepiadaceae) on Acetaminophen-Induced Toxicity in Mice: In Vivo Study and Characterization of Bioactive Compounds through Molecular Docking Approaches. BioMed Research International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1133434
American Medical Association (AMA)
Galani, Borris R. T.& Owona, Brice A.& Chuisseu, Dieudonné P. D.& Machewere, Esaïe& Ngantchouko, Claude B. N.& Moundipa, Paul F.. Hepatoprotective Activity of Leptadenia hastata (Asclepiadaceae) on Acetaminophen-Induced Toxicity in Mice: In Vivo Study and Characterization of Bioactive Compounds through Molecular Docking Approaches. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1133434
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133434