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Metallo-Beta-Lactamase-Producing Acinetobacter spp. from Clinical Isolates at a Tertiary Care Hospital in Ghana
Joint Authors
Obeng, Forson Akua
Olu-Taiwo, Michael
Opintan, Japheth A.
Codjoe, Francis Samuel
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Metallo-beta-lactamase-producing Acinetobacter spp.
is a major challenge for therapeutic treatment of nosocomial infections.
This study is aimed at determining the prevalence of MBL-producing Acinetobacter spp.
among 87 clinical isolates of Acinetobacter spp.
from the Korle-Bu Teaching Hospital, Accra, between August 2014 and July 2015.
Acinetobacter spp.
was identified by standard bacteriological method, and resistance to different antibiotics was assessed with the Kirby–Bauer disc diffusion method.
Meropenem-resistant Acinetobacter isolates were screened for enzyme activity using the modified Hodge test (MHT) and combined disc test (CDT).
Additionally, multiplex PCR was used to determine MBL genes presence (blaVIM,blaIMP, and blaNDM).
All Acinetobacter isolates showed high resistance to cefotaxime (90.8%), ceftazidime (75.9%), cotrimoxazole (70.1%), ciprofloxacin (64.4%), gentamicin (72.4%), levofloxacin (67.8%), and meropenem (59.8%).
A total of 54 (62.1%) of Acinetobacter isolates were multidrug-resistant.
Out of 52 (59.8%) meropenem-resistant Acinetobacter, 3 (5.8%) were carbapenemase producers by MHT, whilst, 23 (44.2%) were CDT positive.
There was no significant difference between the resistance pattern of amikacin, ceftazidime, cotrimoxazole, ciprofloxacin, and meropenem amongst CDT-positive and CDT-negative isolates (p>0.05).
A total of 7/87 (8.1%) CDT-positive Acinetobacter isolates harboured blaNDM; of these, 4 (57.1%) were from wound swabs, urine (n=2) (28.6%), and ear swab (n=1) (14.3%).
The study revealed that less than 9% of Acinetobacter spp.
contained blaNDM encoding genes.
Strict antibiotics usage plan and infection control measures are required to prevent the spread of these resistance genes.
American Psychological Association (APA)
Olu-Taiwo, Michael& Opintan, Japheth A.& Codjoe, Francis Samuel& Obeng, Forson Akua. 2020. Metallo-Beta-Lactamase-Producing Acinetobacter spp. from Clinical Isolates at a Tertiary Care Hospital in Ghana. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1133472
Modern Language Association (MLA)
Olu-Taiwo, Michael…[et al.]. Metallo-Beta-Lactamase-Producing Acinetobacter spp. from Clinical Isolates at a Tertiary Care Hospital in Ghana. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1133472
American Medical Association (AMA)
Olu-Taiwo, Michael& Opintan, Japheth A.& Codjoe, Francis Samuel& Obeng, Forson Akua. Metallo-Beta-Lactamase-Producing Acinetobacter spp. from Clinical Isolates at a Tertiary Care Hospital in Ghana. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1133472
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133472