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A Simple Method for the Design and Development of Flavivirus NS1 Recombinant Proteins Using an In Silico Approach
Joint Authors
Oh, Eun-Jee
Lee, Dong-Gun
Park, Chulmin
Kim, Won-Bok
Cho, Sung-Yeon
Lee, Hyeyoung
Kang, Kyungjoon
Lee, Yoonsuk
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-13
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Even in countries that are currently not facing a flavivirus epidemic, the spread of mosquito-borne flaviviruses presents an increasing public threat, owing to climate change, international travel, and other factors.
Many of these countries lack the resources (viral strains, clinical specimens, etc.) needed for the research that could help cope with the threat imposed by flaviviruses, and therefore, an alternative approach is needed.
Using an in silico approach to global databases, we aimed to design and develop flavivirus NS1 recombinant proteins with due consideration towards antigenic variation.
NS1 genes analyzed in this study included a total of 6,823 sequences, from Dengue virus (DENV), Japanese encephalitis virus (JEV), West Nile virus (WNV), Zika virus (ZIKV), and Yellow fever virus (YKV).
We extracted and analyzed 316 DENV NS1 sequence types (STs), 59 JEV STs, 75 WNV STs, 30 YFV STs, and 43 ZIKV STs using a simple algorithm based on phylogenetic analysis.
STs were reclassified according to the variation of the major epitope by MHC II binding.
78 DENV epitope type (EpT), 29 JEV EpTs, 29 WNV EpTs, 12 YFV EpTs, and 5 ZIKV EpTs were extracted according to their major epitopes.
Also, frequency results showed that there were dominant EpTs in all flavivirus.
Fifteen STs were selected and purified for the expression of recombinant antigen in Escherichia coli by sodium dodecyl sulfate extraction.
Our study details a novel in silico approach for the development of flavivirus diagnostics, including a simple way to screen the important peptide regions.
American Psychological Association (APA)
Park, Chulmin& Kim, Won-Bok& Cho, Sung-Yeon& Oh, Eun-Jee& Lee, Hyeyoung& Kang, Kyungjoon…[et al.]. 2020. A Simple Method for the Design and Development of Flavivirus NS1 Recombinant Proteins Using an In Silico Approach. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1133498
Modern Language Association (MLA)
Park, Chulmin…[et al.]. A Simple Method for the Design and Development of Flavivirus NS1 Recombinant Proteins Using an In Silico Approach. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1133498
American Medical Association (AMA)
Park, Chulmin& Kim, Won-Bok& Cho, Sung-Yeon& Oh, Eun-Jee& Lee, Hyeyoung& Kang, Kyungjoon…[et al.]. A Simple Method for the Design and Development of Flavivirus NS1 Recombinant Proteins Using an In Silico Approach. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1133498
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133498