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Structural Study of Heterogeneous Biological Samples by Cryoelectron Microscopy and Image Processing
Joint Authors
Orlova, Elena
White, H. E.
Ignatiou, A.
Clare, D. K.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-23, 23 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-15
Country of Publication
Egypt
No. of Pages
23
Main Subjects
Abstract EN
In living organisms, biological macromolecules are intrinsically flexible and naturally exist in multiple conformations.
Modern electron microscopy, especially at liquid nitrogen temperatures (cryo-EM), is able to visualise biocomplexes in nearly native conditions and in multiple conformational states.
The advances made during the last decade in electronic technology and software development have led to the revelation of structural variations in complexes and also improved the resolution of EM structures.
Nowadays, structural studies based on single particle analysis (SPA) suggests several approaches for the separation of different conformational states and therefore disclosure of the mechanisms for functioning of complexes.
The task of resolving different states requires the examination of large datasets, sophisticated programs, and significant computing power.
Some methods are based on analysis of two-dimensional images, while others are based on three-dimensional studies.
In this review, we describe the basic principles implemented in the various techniques that are currently used in the analysis of structural conformations and provide some examples of successful applications of these methods in structural studies of biologically significant complexes.
American Psychological Association (APA)
White, H. E.& Ignatiou, A.& Clare, D. K.& Orlova, Elena. 2017. Structural Study of Heterogeneous Biological Samples by Cryoelectron Microscopy and Image Processing. BioMed Research International،Vol. 2017, no. 2017, pp.1-23.
https://search.emarefa.net/detail/BIM-1133600
Modern Language Association (MLA)
White, H. E.…[et al.]. Structural Study of Heterogeneous Biological Samples by Cryoelectron Microscopy and Image Processing. BioMed Research International No. 2017 (2017), pp.1-23.
https://search.emarefa.net/detail/BIM-1133600
American Medical Association (AMA)
White, H. E.& Ignatiou, A.& Clare, D. K.& Orlova, Elena. Structural Study of Heterogeneous Biological Samples by Cryoelectron Microscopy and Image Processing. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-23.
https://search.emarefa.net/detail/BIM-1133600
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133600