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FITC-Labeled Alendronate as an In Vivo Bone pH Sensor
Joint Authors
Song, Jinlin
Li, Yuzhou
Fu, Yiru
Zhang, He
Yang, Sheng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
pH is a critical indicator of bone physiological function and disease status; however, noninvasive and real-time sensing of bone pH in vivo has been a challenge.
Here, we synthesized a bone pH sensor by labeling alendronate with the H+-sensitive dye fluorescein isothiocyanate (Aln-FITC).
Aln-FITC showed selective affinity for hydroxyapatite (HAp) rather than other calcium materials.
An in vivo biodistribution study showed that Aln-FITC can be rapidly and specifically delivered to rat bones after caudal vein injection, and the fluorescence lasted for at least 12 h.
The fluorescence intensity of Aln-FITC binding to HAp linearly decreased when the pH changed from 6 to 12.
This finding was further confirmed on bone blocks and perfused bone when the pH changed from 6.8 to 7.4, indicating unique pH-responsive characteristics in the bone microenvironment.
Aln-FITC was then preliminarily applied to evaluate the changes in bone pH in a nude mouse acidosis model.
Our results demonstrated that Aln-FITC might have the potential for minimally invasive and real-time in vivo bone pH sensing in preclinical studies of bone healing, metabolism, and cancer mechanisms.
American Psychological Association (APA)
Li, Yuzhou& Fu, Yiru& Zhang, He& Song, Jinlin& Yang, Sheng. 2020. FITC-Labeled Alendronate as an In Vivo Bone pH Sensor. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133604
Modern Language Association (MLA)
Li, Yuzhou…[et al.]. FITC-Labeled Alendronate as an In Vivo Bone pH Sensor. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1133604
American Medical Association (AMA)
Li, Yuzhou& Fu, Yiru& Zhang, He& Song, Jinlin& Yang, Sheng. FITC-Labeled Alendronate as an In Vivo Bone pH Sensor. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133604
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133604