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Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats
Joint Authors
Wang, Ling
Liu, Meng-ying
Wang, Da-Bo
Yao, Ru-Yong
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
Using rat conjunctival bleb model, we correlated changes morphology and histology in the bleb with changes in MMP-2 and TIMP-2 levels.
Methods.
Filtering surgeries were performed on rats.
Dynamic changes in morphology and histopathology were observed using HE staining.
Expression of MMP-2 and TIMP-2 was determined by immunofluorescence microscopy and western blotting.
Results.
Well-elevated filtering blebs formed and persisted for an average of 12 days.
Histological examination showed that inflammatory was dominant in postoperative days 1–3, and proliferating manifestation became the main sign 5 days later.
Western blot showed that MMP-2 was downregulated 1 day after surgery, upregulated at 3 days, and observed with a peak at 7 days; then it persisted until 28 days.
The difference was statistically significant (F = 280.18, p<0.01).TIMP-2 was upregulated 1 day after surgery and observed with a peak at 5 days; then it persisted until 28 days.
The difference was statistically significant (F = 145.34, p<0.01).
Conclusions.
During the processes of conjunctival filtering bleb and scar formation in rats, the changes in MMP-2 and TIMP-2 levels in the filtering area, together with a corresponding proliferation of fibroblasts and the accumulation of collagen fibres, resulted in scarring of filtering blebs.
American Psychological Association (APA)
Wang, Ling& Wang, Da-Bo& Liu, Meng-ying& Yao, Ru-Yong. 2017. Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1133629
Modern Language Association (MLA)
Wang, Ling…[et al.]. Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1133629
American Medical Association (AMA)
Wang, Ling& Wang, Da-Bo& Liu, Meng-ying& Yao, Ru-Yong. Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1133629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133629