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Feeder Cell Type Affects the Growth of In Vitro Cultured Bovine Trophoblast Cells
Joint Authors
Saadeldin, Islam M.
Abdelfattah-Hassan, Ahmed
Swelum, Ayman Abdel-Aziz
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Trophectoderm cells are the foremost embryonic cells to differentiate with prospective stem-cell properties.
In the current study, we aimed at improving the current approach for trophoblast culture by using granulosa cells as feeders.
Porcine granulosa cells (PGCs) compared to the conventional mouse embryonic fibroblasts (MEFs) were used to grow trophectoderm cells from hatched bovine blastocysts.
Isolated trophectoderm cells were monitored and displayed characteristic epithelial/cuboidal morphology.
The isolated trophectoderm cells expressed mRNA of homeobox protein (CDX2), cytokeratin-8 (KRT8), and interferon tau (IFNT).
The expression level was higher on PGCs compared to MEFs throughout the study.
In addition, primary trophectoderm cell colonies grew faster on PGCs, with a doubling time of approximately 48 hrs, compared to MEFs.
PGCs feeders produced a fair amount of 17β-estradiol and progesterone.
We speculated that the supplementation of sex steroids and still-unknown factors during the trophoblasts coculture on PGCs have helped to have better trophectoderm cell’s growth than on MEFs.
This is the first time to use PGCs as feeders to culture trophectoderm cells and it proved superior to MEFs.
We propose PGCs as alternative feeders for long-term culture of bovine trophectoderm cells.
This model will potentially benefit studies on the early trophoblast and embryonic development in bovines.
American Psychological Association (APA)
Saadeldin, Islam M.& Abdelfattah-Hassan, Ahmed& Swelum, Ayman Abdel-Aziz. 2017. Feeder Cell Type Affects the Growth of In Vitro Cultured Bovine Trophoblast Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1133646
Modern Language Association (MLA)
Saadeldin, Islam M.…[et al.]. Feeder Cell Type Affects the Growth of In Vitro Cultured Bovine Trophoblast Cells. BioMed Research International No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1133646
American Medical Association (AMA)
Saadeldin, Islam M.& Abdelfattah-Hassan, Ahmed& Swelum, Ayman Abdel-Aziz. Feeder Cell Type Affects the Growth of In Vitro Cultured Bovine Trophoblast Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1133646
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133646