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MicroRNA Expression Varies according to Glucose Tolerance, Measurement Platform, and Biological Source
Joint Authors
Pheiffer, Carmen
Dias, S.
Hemmings, S.
Muller, C.
Louw, Johan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Dysregulated microRNA (miRNA) expression is observed during type 2 diabetes (T2D), although the consistency of miRNA expression across measurement platform and biological source is uncertain.
Here we report miRNA profiling in the whole blood and serum of South African women with different levels of glucose tolerance, using next generation sequencing (NGS) and quantitative real time PCR (qRT-PCR).
Whole blood-derived miRNAs from women with newly diagnosed T2D (n=4), impaired glucose tolerance (IGT) (n=4), and normal glucose tolerance (NGT) (n=4) were subjected to NGS, whereafter transcript levels of selected miRNAs were quantified in the whole blood and serum of these women using qRT-PCR.
Of the five significantly differentially expressed miRNAs identified by NGS, only the directional increase of miR-27b in women with IGT compared to NGT was confirmed in whole blood and serum, using qRT-PCR.
Functional enrichment of miR-27b gene targets identified biological pathways associated with glucose transport and insulin regulation.
In conclusion, this study showed poor correlation in miRNA expression profiled using NGS and qRT-PCR and in whole blood and serum.
The consistent increased expression of miR-27b in women with IGT compared to NGT across measurement platform and biological source holds potential as a biomarker for risk stratification in our population.
American Psychological Association (APA)
Dias, S.& Hemmings, S.& Muller, C.& Louw, Johan& Pheiffer, Carmen. 2017. MicroRNA Expression Varies according to Glucose Tolerance, Measurement Platform, and Biological Source. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1133676
Modern Language Association (MLA)
Dias, S.…[et al.]. MicroRNA Expression Varies according to Glucose Tolerance, Measurement Platform, and Biological Source. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1133676
American Medical Association (AMA)
Dias, S.& Hemmings, S.& Muller, C.& Louw, Johan& Pheiffer, Carmen. MicroRNA Expression Varies according to Glucose Tolerance, Measurement Platform, and Biological Source. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1133676
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133676