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Evaluation of Two Supplemented Culture Media for Long-Term, Room-Temperature Preservation of Streptococcus pneumoniae Strains
Joint Authors
Quintero Moreno, Beatriz
Mendoza, Evelyn
Araque, María
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To produce two supplemented agar types in order to store pneumococci for several months at room temperature.
Methods.
Todd-Hewitt/Hemoglobin/Yeast/Charcoal/Agar (TH-HYC) and Todd-Hewitt/Skim-Milk/Yeast/Charcoal/Agar (TH-SYC) were used to prepare two supplemented agar types.
Nineteen pneumococci isolated from patients or asymptomatic carriers displaying diverse serotypes and multilocus sequence types (MLST) were subcultured and stored onto supplemented agar types, in four different tests, at room temperature.
Findings.
At the end of all tests (4–6 months) all noncontaminated subcultures were viable and maintained all phenotypic characteristics.
Survival-time curves revealed a slow decrease of viable CFU over time on agar types, but at the end the number of viable CFU was satisfactory (≥2+ of growth).
Decreasing of CFU was significantly higher for clinical versus nasopharyngeal isolates.
Subcultures contamination rates were 6.25% and 14.58% after 2 and 6 months of storage, respectively.
Conclusion.
TH-HYC and TH-SYC agar types allowed the viability of pneumococci with several serotypes, MLST, and genetic profiles, after 6 months of storage at room temperature.
We consider that these agar types are a valid alternative to preserve pneumococci over an extended period, especially when methods as cryopreservation or lyophilization are not available, and are useful for transporting strains between laboratories.
American Psychological Association (APA)
Quintero Moreno, Beatriz& Araque, María& Mendoza, Evelyn. 2017. Evaluation of Two Supplemented Culture Media for Long-Term, Room-Temperature Preservation of Streptococcus pneumoniae Strains. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1133725
Modern Language Association (MLA)
Quintero Moreno, Beatriz…[et al.]. Evaluation of Two Supplemented Culture Media for Long-Term, Room-Temperature Preservation of Streptococcus pneumoniae Strains. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1133725
American Medical Association (AMA)
Quintero Moreno, Beatriz& Araque, María& Mendoza, Evelyn. Evaluation of Two Supplemented Culture Media for Long-Term, Room-Temperature Preservation of Streptococcus pneumoniae Strains. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1133725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133725