Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Naïve Schwann Cells in the Sciatic Nerve Injury Model
Joint Authors
Chi, Guangfan
Zhang, Mingzi
Jiang, Mei Hua
Kim, Dae-Wook
Ahn, Woosung
Chung, Eunkyung
Son, Youngsook
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Purpose.
The fate and function of the induced Schwann cells (iSCs) like cells from adipose tissue have not been critically evaluated in vivo after transplantation.
The objective of this study is to compare the fate of iSCs with naïve SCs (nSCs) after transplantation into the lesion sites of sciatic nerve, respectively.
Methods.
Adipose-derived stem cells from eGFP-expressing transgenic rat’s subcutaneous fat were induced to iSCs in vitro.
iSCs were injected to the sciatic nerve lesion area after crush injury and the cells fate was comparatively analyzed with that of nSCs from the same rat.
Results.
At 12 weeks after transplantation, nSCs were detected only in the restricted area of cell transplantation site but iSCs were widely distributed all over the sciatic nerve.
Based on double fluorescence observations, both iSCs and naïve ones were colocalized with P0-expressing myelin sheath, outbound by laminin-expressing basal membrane, and terminated at contactin-associated protein-expressing doublets.
However, some of iSCs were also differentiated to the fibrocyte/fibroblast-like cells.
In the histological analysis of repaired sciatic nerves, axon density was higher in iSC-received group than in the nSCs group and normal sciatic nerve.
Conclusion.
iSCs induced from subcutaneous fat tissues have higher engraftment and migration capacity than nSCs.
American Psychological Association (APA)
Zhang, Mingzi& Jiang, Mei Hua& Kim, Dae-Wook& Ahn, Woosung& Chung, Eunkyung& Son, Youngsook…[et al.]. 2017. Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Naïve Schwann Cells in the Sciatic Nerve Injury Model. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1133744
Modern Language Association (MLA)
Zhang, Mingzi…[et al.]. Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Naïve Schwann Cells in the Sciatic Nerve Injury Model. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1133744
American Medical Association (AMA)
Zhang, Mingzi& Jiang, Mei Hua& Kim, Dae-Wook& Ahn, Woosung& Chung, Eunkyung& Son, Youngsook…[et al.]. Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Naïve Schwann Cells in the Sciatic Nerve Injury Model. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1133744
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133744