Pentoxifylline Regulates Plasminogen Activator Inhibitor-1 Expression and Protein Kinase A Phosphorylation in Radiation-Induced Lung Fibrosis
Joint Authors
Park, Sunhoo
Jin, Young Woo
Lee, Jong Geol
Kim, Kyeong Min
Shim, Sehwan
Kim, Min-Jung
Myung, Jae Kyung
Jang, Won-Suk
Bae, Chang-Hwan
Lee, Sun-Joo
Lee, Seung-Sook
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
Radiation-induced lung fibrosis (RILF) is a serious late complication of radiotherapy.
In vitro studies have demonstrated that pentoxifylline (PTX) has suppressing effects in extracellular matrix production in fibroblasts, while the antifibrotic action of PTX alone using clinical dose is yet unexplored.
Materials and Methods.
We used micro-computed tomography (micro-CT) and histopathological analysis to evaluate the antifibrotic effects of PTX in a rat model of RILF.
Results.
Micro-CT findings showed that lung density, volume loss, and mediastinal shift are significantly increased at 16 weeks after irradiation.
Simultaneously, histological analysis demonstrated thickening of alveolar walls, destruction of alveolar structures, and excessive collagen deposition in the irradiated lung.
PTX treatment effectively attenuated the fibrotic changes based on both micro-CT and histopathological analyses.
Western analysis also revealed increased levels of plasminogen activator inhibitor- (PAI-) 1 and fibronectin (FN) and PTX treatment reduced expression of PAI-1 and FN by restoring protein kinase A (PKA) phosphorylation but not TGF-β/Smad in both irradiated lung tissues and epithelial cells.
Conclusions.
Our results demonstrate the antifibrotic effect of PTX on radiation-induced lung fibrosis and its effect on modulation of PKA and PAI-1 expression as possible antifibrotic mechanisms.
American Psychological Association (APA)
Lee, Jong Geol& Shim, Sehwan& Kim, Min-Jung& Myung, Jae Kyung& Jang, Won-Suk& Bae, Chang-Hwan…[et al.]. 2017. Pentoxifylline Regulates Plasminogen Activator Inhibitor-1 Expression and Protein Kinase A Phosphorylation in Radiation-Induced Lung Fibrosis. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1133794
Modern Language Association (MLA)
Lee, Jong Geol…[et al.]. Pentoxifylline Regulates Plasminogen Activator Inhibitor-1 Expression and Protein Kinase A Phosphorylation in Radiation-Induced Lung Fibrosis. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1133794
American Medical Association (AMA)
Lee, Jong Geol& Shim, Sehwan& Kim, Min-Jung& Myung, Jae Kyung& Jang, Won-Suk& Bae, Chang-Hwan…[et al.]. Pentoxifylline Regulates Plasminogen Activator Inhibitor-1 Expression and Protein Kinase A Phosphorylation in Radiation-Induced Lung Fibrosis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1133794
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133794