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PD-L1 Promotes Self-Renewal and Tumorigenicity of Malignant Melanoma Initiating Cells
Joint Authors
Zheng, Fang
Dang, Jianzhong
Zha, Hui
Zhang, Bingyu
Lin, Ming
Cheng, Fanjun
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Recent studies have indicated that therapeutic antibodies targeting PD-L1 show remarkable efficacy in clinical trials in multiple tumors and that a melanoma cell-intrinsic PD-1: PD-L1 axis promotes tumor growth.
However, few studies have shown tumor-intrinsic PD-L1 effects in malignant melanoma initiating cells (MMICs).
Here, we aim to determine the possible regulatory effects of PD-L1 on MMICs.
The ALDEFLUOR kit was used to identify ALDH+ MMICs.
Flow cytometry was used to examine the expression of PD-L1 on ALDH+ MMICs.
To determine the role of PD-L1 in MMICs self-renewal, we cultured melanoma cells with anti-PD-L1 and measured tumorsphere formation and apoptosis.
In addition, the effects of anti-PD-L1 on tumorigenicity and residual ALDH+ MMICs in tumors were evaluated in vivo.
We demonstrated that melanoma cell-intrinsic PD-L1 was expressed in ALDH+ MMICs.
Blocking PD-L1 in melanoma cell lines impaired tumorsphere formation and induced the apoptosis of sphere cells.
In addition, blocking PD-L1 inhibited tumor growth in vivo.
We observed residual ALDH+ MMICs within the tumor.
The results showed that blocking PD-L1 also significantly decreased the residual ALDH+ MMICs in the tumors.
In conclusion, these results suggest a new mechanism underlying melanoma progression and PD-L1-targeted therapy, which is distinct from the immunomodulatory actions of PD-L1.
American Psychological Association (APA)
Zheng, Fang& Dang, Jianzhong& Zha, Hui& Zhang, Bingyu& Lin, Ming& Cheng, Fanjun. 2017. PD-L1 Promotes Self-Renewal and Tumorigenicity of Malignant Melanoma Initiating Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1133814
Modern Language Association (MLA)
Zheng, Fang…[et al.]. PD-L1 Promotes Self-Renewal and Tumorigenicity of Malignant Melanoma Initiating Cells. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1133814
American Medical Association (AMA)
Zheng, Fang& Dang, Jianzhong& Zha, Hui& Zhang, Bingyu& Lin, Ming& Cheng, Fanjun. PD-L1 Promotes Self-Renewal and Tumorigenicity of Malignant Melanoma Initiating Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1133814
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133814