Resistance to Chronic Toxoplasma gondii Infection Induced by a DNA Vaccine Expressing GRA16
Joint Authors
Zhang, Nian-Zhang
Zhu, Xing-Quan
Hu, Ling-Ying
Zhang, Fu-Kai
Wang, Meng
Gao, Qi
Wang, Jin-Lei
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Toxoplasma gondii can infect all warm-blooded animals including human beings.
T.
gondii dense granule protein 16 (TgGRA16) as a crucial virulence factor could modulate the host gene expression.
Here, a DNA vaccine expressing TgGRA16 was constructed to explore the protective efficacy against T.
gondii infection in Kunming mice.
The immune responses induced by pVAX-GRA16 were also evaluated.
Mice immunized with pVAX-GRA16 could elicit higher levels of specific IgG antibody and strong cellular response compared to those in controls.
The DNA vaccination significantly increased the levels of cytokines (IFN-γ, IL-2, IL-4, and IL-10) and the percentages of CD4+ and CD8+ T cells in mice.
After lethal challenge, mice immunized with pVAX-GRA16 (8.4±0.78 days) did not show a significant longer survival time than that in controls (7.1±0.30 days) (p>0.05).
However, in chronic toxoplasmosis model (administration of 10 brain cysts of PRU strain orally), numbers of tissue cysts in mice immunized with pVAX-GRA16 were significantly reduced compared to those in controls (p<0.05) and the rate of reduction could reach 43.89%.
The results indicated that the TgGRA16 would be a promising vaccine candidate for further development of effective epitope-based vaccines against chronic T.
gondii infection in mice.
American Psychological Association (APA)
Hu, Ling-Ying& Zhang, Nian-Zhang& Zhang, Fu-Kai& Wang, Meng& Gao, Qi& Wang, Jin-Lei…[et al.]. 2017. Resistance to Chronic Toxoplasma gondii Infection Induced by a DNA Vaccine Expressing GRA16. BioMed Research International،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1133816
Modern Language Association (MLA)
Hu, Ling-Ying…[et al.]. Resistance to Chronic Toxoplasma gondii Infection Induced by a DNA Vaccine Expressing GRA16. BioMed Research International No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1133816
American Medical Association (AMA)
Hu, Ling-Ying& Zhang, Nian-Zhang& Zhang, Fu-Kai& Wang, Meng& Gao, Qi& Wang, Jin-Lei…[et al.]. Resistance to Chronic Toxoplasma gondii Infection Induced by a DNA Vaccine Expressing GRA16. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1133816
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133816