Overexpression of Ubiquinol-Cytochrome c Reductase Core Protein 1 May Protect H9c2 Cardiac Cells by Binding with Zinc
Joint Authors
Yi, Tingting
Wu, Xiaoxiao
Long, Zonghong
Duan, Guangyou
Wu, Zhuoxi
Li, Hong
Chen, Huifang
Zhou, Xiaoying
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In several recent studies, proteomics analyses suggest that increase of ubiquinol-cytochrome c reductase core protein 1 (UQCRC1) is cardio-protective.
However, direct evidence for this effect has not yet been obtained.
Thus, the current study aimed to determine this effect and the mechanism underlying this effect.
The results showed that overexpression of UQCRC1 protected H9c2 cardiac cells against in vitro simulated ischemia-reperfusion by maintaining mitochondrial membrane potential and suppressing the expression of caspase-3.
These protective effects were significantly enhanced by exogenous Zn2+ but completely abolished by Zn2+-selective chelator TPEN.
Furthermore, the upregulation of UQCRC1 reduced the concentration of free Zn2+ in mitochondria, whereas the downregulation of UQCRC1 increased the concentration of free Zn2+ in mitochondria.
In conclusion, the overexpression of UQCRC1 can protect H9c2 cardiac cells against simulated ischemia/reperfusion, and this cardio-protective effect is likely mediated by zinc binding.
American Psychological Association (APA)
Yi, Tingting& Wu, Xiaoxiao& Long, Zonghong& Duan, Guangyou& Wu, Zhuoxi& Li, Hong…[et al.]. 2017. Overexpression of Ubiquinol-Cytochrome c Reductase Core Protein 1 May Protect H9c2 Cardiac Cells by Binding with Zinc. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1133835
Modern Language Association (MLA)
Yi, Tingting…[et al.]. Overexpression of Ubiquinol-Cytochrome c Reductase Core Protein 1 May Protect H9c2 Cardiac Cells by Binding with Zinc. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1133835
American Medical Association (AMA)
Yi, Tingting& Wu, Xiaoxiao& Long, Zonghong& Duan, Guangyou& Wu, Zhuoxi& Li, Hong…[et al.]. Overexpression of Ubiquinol-Cytochrome c Reductase Core Protein 1 May Protect H9c2 Cardiac Cells by Binding with Zinc. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1133835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133835