Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5aCa2+ Signaling In Vitro
Joint Authors
Yu, Lai
Liu, Shuitao
Zhao, Zhe
Xia, Lin
Zhang, Haochong
Lou, Jing
Yang, Jun
Xing, Gengmei
Xing, Gengyan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
This study aimed to identify the optimal extracorporeal shock wave (ESW) intensity and to investigate its effect on subchondral bone rebuilt in vivo and Wnt5a/Ca2+ signaling in vitro using an osteoarthritis (OA) rat model and bone marrow mesenchymal stem cells (BMMSCs), respectively.
Methods.
OA rats treated with (OA + ESW group) or without (OA group) ESW (n=12/group) were compared with healthy controls (control group, n=12).
Gait patterns and subchondral trabecular bone changes were measured.
Western blot and quantitative real-time polymerase chain reaction detected protein expression and gene transcription, respectively.
Results.
The gait disturbances of OA + ESW group were significantly improved compared with the OA group at 6th and 8th weeks.
The micro-CT analysis indicated that the BMD, BSV/BV, BV/TV, Tr.S, and Tr.Th are significantly different between OA group and OA + ESW group.
Expression of Wnt5a was increased rapidly after ESW treatment at 0.6 bar and peaked after 30 min.
Conclusions.
ESW were positive for bone remodeling in joint tibial condyle subchondral bone of OA rat.
ESW prevented histological changes in OA and prevented gait disturbance associated with OA progression.
Optimal intensity of ESW induced changes in BMMSCs via activation of the Wnt5a/Ca2+ signaling pathway.
American Psychological Association (APA)
Yu, Lai& Liu, Shuitao& Zhao, Zhe& Xia, Lin& Zhang, Haochong& Lou, Jing…[et al.]. 2017. Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5aCa2+ Signaling In Vitro. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1133885
Modern Language Association (MLA)
Yu, Lai…[et al.]. Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5aCa2+ Signaling In Vitro. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1133885
American Medical Association (AMA)
Yu, Lai& Liu, Shuitao& Zhao, Zhe& Xia, Lin& Zhang, Haochong& Lou, Jing…[et al.]. Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5aCa2+ Signaling In Vitro. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1133885
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133885