Knockdown of Matrix Metallopeptidase 9 Inhibits Metastasis of Oral Squamous Cell Carcinoma Cells in a Zebrafish Xenograft Model
Joint Authors
Wen, Jinlin
Yin, Panpan
Li, Linwei
Kang, Guihua
Ning, Guozhu
Cao, Yu
Gao, Feng
Su, Ying
Wu, Yanlin
Zhang, Xinyan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Destruction of extracellular matrix (ECM) is one of the basic steps of tumor invasion and metastasis.
Matrix metalloproteinase (MMP) 9, a kind of zinc-ion-dependent endopeptidase, can degrade almost all protein components in the ECM, destroy the histological barrier of tumor cell invasion, and play a key role in tumor invasion and metastasis.
The role of MMP-9 in tumor invasion and metastasis has attracted increasing attention and is considered the main proteolytic enzyme in this process.
Although the overexpression of MMP-9 was detected in Oral squamous cell carcinoma (OSCC) tissues, further basic studies in vivo and in vitro are needed to investigate the role of MMP-9 in OSCCs and provide scientific validation.
In this research, we developed a novel OSCC zebrafish xenograft model to study the role of the MMP-9 gene in oral carcinogenesis.
Firstly, the MMP-9/shRNA lentiviral clone and control virus were constructed and transfected into OSCC cells.
Then, the decreasing expression of MMP-9 was verified by RT-PCR and immunocytochemistry.
Cell proliferation was detected by MTT assay.
Colony formation was evaluated by colony formation assay.
Cell invasion was evaluated using transwell invasion assay in vitro.
In addition, OSCC cells with MMP-9/shRNA knockdown and control vector were injected into zebrafish and an OSCC tumor model in zebrafish was established to evaluate invasion and metastasis in vivo.
Knockdown of MMP-9 gene by shRNA could inhibit OSCC cell growth and clone formation and markedly suppress cell invasion in vitro.
And the knockdown of the MMP-9 gene could also significantly decrease the metastatic distance and number of metastatic tumor cells or lesions in vivo and suppress the metastasis rate in xenografted zebrafish.
Taken together, these evidences indicated that the knockdown of MMP-9 might suppress OSCC cell invasion and metastasis in vivo and in vitro.
The MMP-9 gene may be a promising therapeutic target for OSCCs in the future.
American Psychological Association (APA)
Wen, Jinlin& Yin, Panpan& Li, Linwei& Kang, Guihua& Ning, Guozhu& Cao, Yu…[et al.]. 2020. Knockdown of Matrix Metallopeptidase 9 Inhibits Metastasis of Oral Squamous Cell Carcinoma Cells in a Zebrafish Xenograft Model. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1133895
Modern Language Association (MLA)
Wen, Jinlin…[et al.]. Knockdown of Matrix Metallopeptidase 9 Inhibits Metastasis of Oral Squamous Cell Carcinoma Cells in a Zebrafish Xenograft Model. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1133895
American Medical Association (AMA)
Wen, Jinlin& Yin, Panpan& Li, Linwei& Kang, Guihua& Ning, Guozhu& Cao, Yu…[et al.]. Knockdown of Matrix Metallopeptidase 9 Inhibits Metastasis of Oral Squamous Cell Carcinoma Cells in a Zebrafish Xenograft Model. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1133895
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133895