Circulating miRNA-3552 as a Potential Biomarker for Ischemic Stroke in Rats
Joint Authors
Dong, Lipeng
Zhao, Jingru
He, Weiliang
Chu, Bao
Zhang, Jijie
Wu, Zongkai
Zhao, Congying
Cheng, Jinming
Yao, Wentao
Wang, Hebo
Li, Litao
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objective.
With the growing incidence of ischemic stroke worldwide, there is an urgent need to identify blood biomarkers for ischemic stroke patients.
Thus, our aim was to identify potential circulating microRNA (miRNA) as a potential biomarker and to explore its potential mechanism for ischemic stroke in rats.
Methods.
The mRNA dataset GSE97537 and miRNA dataset GSE97532 were downloaded from the Gene Expression Omnibus (GEO) GSE97537 including 7 middle cerebral artery occlusion (MCAO) rat brain tissues and 5 sham-operated rat brain tissues GSE97532 including 6 MCAO rat blood samples and 3 sham-operated rat blood samples.
Differentially expressed mRNAs and miRNAs with corrected p value ≤ 0.01 and fold change ≥2 or ≤0.05 were identified.
To explore potential biological processes and pathways of differentially expressed mRNAs, functional enrichment analysis was performed.
The target mRNAs of differentially expressed miRNAs were predicted using DNA Intelligent Analysis (DIANA)-microT tools.
The target mRNAs and differentially expressed mRNAs were intersected.
Results.
1228 differentially expressed mRNAs in MCAO rat brain tissues were identified.
Highly expressed mRNAs were mainly enriched in the inflammatory responses.
Nine differentially expressed miRNAs were identified in MCAO rat blood samples.
A total of 673 target mRNAs were predicted to significantly bind these differentially expressed miRNAs.
Among them, 54 target mRNAs were differentially expressed in MCAO rat blood samples.
Enrichment analysis results showed that these 54 target mRNAs were closely related to neurological diseases and immune responses.
Among all miRNA-mRNA relationship, miR-3552-CASP3 interaction was identified, indicating that CASP3 might be mediated by miR-3552.
Functional enrichment analysis revealed that CASP3 was involved in the apoptosis pathway, indicating that miR-3552 might participate in apoptosis by CASP3.
Conclusion.
Our findings reveal that circulating miR-3552 shows promise as a potential biomarker for ischemic stroke in rats.
American Psychological Association (APA)
Li, Litao& Dong, Lipeng& Zhao, Jingru& He, Weiliang& Chu, Bao& Zhang, Jijie…[et al.]. 2020. Circulating miRNA-3552 as a Potential Biomarker for Ischemic Stroke in Rats. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1133932
Modern Language Association (MLA)
Li, Litao…[et al.]. Circulating miRNA-3552 as a Potential Biomarker for Ischemic Stroke in Rats. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1133932
American Medical Association (AMA)
Li, Litao& Dong, Lipeng& Zhao, Jingru& He, Weiliang& Chu, Bao& Zhang, Jijie…[et al.]. Circulating miRNA-3552 as a Potential Biomarker for Ischemic Stroke in Rats. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1133932
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133932