Chenodeoxycholic Acid Derivative HS-1200 Inhibits Hepatocarcinogenesis and Improves Liver Function in Diethylnitrosamine-Exposed Rats by Downregulating MTH1

Abstract EN

Aim.

To investigate the effects of HS-1200 on liver tumorigenesis and liver function in a diethylnitrosamine- (DEN-) induced hepatocellular carcinoma (HCC) rat model.

Methods.

Rats were randomly assigned into five groups: control, HS-1200, HCC, HCC + low dose HS-1200, and HCC + high dose HS-1200 groups.

Rat HCC model was established by intraperitoneal injection of DEN.

And rats were given HS-1200 by daily oral gavage.

After 20 weeks, we examined animal body weight, liver weight, liver pathological changes, serum levels of AST, ALT, and AFP, and mutT homologue gene 1 (MTH1) in liver tissue.

Results.

Oral gavage of HS-1200 significantly increased animal body weight and decreased liver weight as well as liver coefficient in HCC rats (P<0.05 versus HCC group).

Moreover, oral administration of HS-1200 suppressed tumorigenesis, attenuated pathological changes in liver tissues, and decreased serum levels of AST, ALT, and AFP in HCC rats (P<0.05 versus HCC group).

In addition, the mRNA level of MTH1 was upregulated in the liver tissues of HCC rats (P<0.05 versus control group), which was reversed by HS-1200 treatment in a dose-dependent manner (P<0.05 versus HCC group).

Conclusions.

HS-1200 inhibits hepatocarcinogenesis and improves liver function maybe by inducing downregulation of MTH1.