The Establishment of Esophageal Precancerous Lesion Model by Using p53 Conditional Knockout Mouse in Esophageal Epithelium
Joint Authors
Dong, Ziming
Zhu, Lili
Xu, Yanyan
Chen, Xinhuan
Qin, Jiace
Niu, Tingting
Zhu, Yanyan
Jiang, Yanan
Zhao, Simin
Liu, Kangdong
Lu, Jing
Jin, Ge
Ma, Junfen
Zhao, Jimin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Understanding the molecular mechanisms of precancerous lesion of esophageal cancer is beneficial for early diagnosis and early treatment.
The deletion of p53 gene is common in esophageal cancer, but its pathogenesis is still unclear.
An animal model is urgently needed to study the mechanisms of esophageal cancer and p53 deficiency.
KO mice (p53flox/flox.ED-L2-Cre+/−) and the corresponding control Loxp mice (p53flox/flox.ED-L2-Cre−/−) were obtained by crossing between the p53flox/flox mice and ED-L2-Cre+/− mice.
Methylbenzylnitrosamine (NMBA) was injected subcutaneously to induce esophageal precancerous lesion of these two groups of mice.
Hematoxylin and eosin staining analysis was performed to evaluate the number and extent of esophageal precancerous lesions in KO mice and Loxp mice at the 16th and 48th weeks.
Immunohistochemistry analysis was used to detect the change of Ki67, P21, Bcl-2, and Bax proteins.
The number and extent of esophageal precancerous lesions in KO mice were significantly increased compared with the control at the 16th and 48th weeks under the induction of NMBA.
The Ki67, P21, Bcl-2, and Bax proteins also had cancer-related pathological characteristics.
These results suggest that the esophageal precancerous lesion model was established under the combined effect of p53 gene deletion in esophageal epithelium and NMBA, which could provide a new esophageal precancerous lesion model to explore the mechanism of precancerous lesions.
American Psychological Association (APA)
Zhu, Lili& Xu, Yanyan& Chen, Xinhuan& Qin, Jiace& Niu, Tingting& Zhu, Yanyan…[et al.]. 2020. The Establishment of Esophageal Precancerous Lesion Model by Using p53 Conditional Knockout Mouse in Esophageal Epithelium. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1133979
Modern Language Association (MLA)
Zhu, Lili…[et al.]. The Establishment of Esophageal Precancerous Lesion Model by Using p53 Conditional Knockout Mouse in Esophageal Epithelium. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1133979
American Medical Association (AMA)
Zhu, Lili& Xu, Yanyan& Chen, Xinhuan& Qin, Jiace& Niu, Tingting& Zhu, Yanyan…[et al.]. The Establishment of Esophageal Precancerous Lesion Model by Using p53 Conditional Knockout Mouse in Esophageal Epithelium. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1133979
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133979