Fibrillar Type I Collagen Enhances the Differentiation and Proliferation of Myofibroblasts by Lowering α2β1 Integrin Expression in Cardiac Fibrosis
Joint Authors
Hong, Jian
Chu, Ming
Qian, Lijun
Wang, Junhong
Guo, Yan
Xu, Di
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Many studies have shown that α2β1 integrin plays an important role in the development of cardiac fibrosis.
However, the mechanism of how α2β1 integrin regulates the differentiation and proliferation of myofibroblasts in cardiac fibrosis through fibrillar collagen (FC) remains uncertain.
We established that FC mimicked the 3-dimensional extracellular matrix (ECM) of fibroblasts from post-myocardial infarction (MI) patients in vivo.
This allowed us to explore the differentiation and proliferation of cardiac fibroblasts on FC.
Here, we report that low expression of α2β1 integrin increased protein kinase B (AKT) activation and α-smooth muscle actin (α-SMA) expression.
This occurred due to the instability of phosphatase and tensin homolog (PTEN) in myofibroblasts on FC.
We also demonstrated that FC reduced protein phosphatase type 2A (PP2A) activity of myofibroblasts, which was coincident with low α2β1 integrin expression and activation of AKT, but not mitogen-activated protein kinase (ERK).
In addition, knock-down of both β1 integrin and PP2A in fibroblasts promoted differentiation and proliferation via AKT activation and increased α-SMA expression.
In summary, our study demonstrated that low α2β1 integrin expression regulated its downstream targets PTEN and AKT via crosstalk with PP2A, a critical cell signaling pathway that permits aberrant differentiation and proliferation of myofibroblasts on FC.
American Psychological Association (APA)
Hong, Jian& Chu, Ming& Qian, Lijun& Wang, Junhong& Guo, Yan& Xu, Di. 2017. Fibrillar Type I Collagen Enhances the Differentiation and Proliferation of Myofibroblasts by Lowering α2β1 Integrin Expression in Cardiac Fibrosis. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1134183
Modern Language Association (MLA)
Hong, Jian…[et al.]. Fibrillar Type I Collagen Enhances the Differentiation and Proliferation of Myofibroblasts by Lowering α2β1 Integrin Expression in Cardiac Fibrosis. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1134183
American Medical Association (AMA)
Hong, Jian& Chu, Ming& Qian, Lijun& Wang, Junhong& Guo, Yan& Xu, Di. Fibrillar Type I Collagen Enhances the Differentiation and Proliferation of Myofibroblasts by Lowering α2β1 Integrin Expression in Cardiac Fibrosis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1134183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134183