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RNA-Based Stable Isotope Probing Suggests Allobaculum spp. as Particularly Active Glucose Assimilators in a Complex Murine Microbiota Cultured In Vitro
Joint Authors
Riedel, Christian U.
Herrmann, Elena
Young, Wayne
Rosendale, Douglas
Reichert-Grimm, Verena
Conrad, Ralf
Egert, Markus
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
RNA-based stable isotope probing (RNA-SIP) and metabolic profiling were used to detect actively glucose-consuming bacteria in a complex microbial community obtained from a murine model system.
A faeces-derived microbiota was incubated under anaerobic conditions for 0, 2, and 4 h with 40 mM [U13C]glucose.
Isopycnic density gradient ultracentrifugation and fractionation of isolated RNA into labeled and unlabeled fractions followed by 16S rRNA sequencing showed a quick adaptation of the bacterial community in response to the added sugar, which was dominated by unclassified Lachnospiraceae species.
Inspection of distinct fractions of isotope-labeled RNA revealed Allobaculum spp.
as particularly active glucose utilizers in the system, as the corresponding RNA showed significantly higher proportions among the labeled RNA.
With time, the labeled sugar was used by a wider spectrum of faecal bacteria.
Metabolic profiling indicated rapid fermentation of [U13C]glucose, with lactate, acetate, and propionate being the principal 13C-labeled fermentation products, and suggested that “cross-feeding” occurred in the system.
RNA-SIP combined with metabolic profiling of 13C-labeled products allowed insights into the microbial assimilation of a general model substrate, demonstrating the appropriateness of this technology to study assimilation processes of nutritionally more relevant substrates, for example, prebiotic carbohydrates, in the gut microbiota of mice as a model system.
American Psychological Association (APA)
Herrmann, Elena& Young, Wayne& Rosendale, Douglas& Reichert-Grimm, Verena& Riedel, Christian U.& Conrad, Ralf…[et al.]. 2017. RNA-Based Stable Isotope Probing Suggests Allobaculum spp. as Particularly Active Glucose Assimilators in a Complex Murine Microbiota Cultured In Vitro. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1134219
Modern Language Association (MLA)
Herrmann, Elena…[et al.]. RNA-Based Stable Isotope Probing Suggests Allobaculum spp. as Particularly Active Glucose Assimilators in a Complex Murine Microbiota Cultured In Vitro. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1134219
American Medical Association (AMA)
Herrmann, Elena& Young, Wayne& Rosendale, Douglas& Reichert-Grimm, Verena& Riedel, Christian U.& Conrad, Ralf…[et al.]. RNA-Based Stable Isotope Probing Suggests Allobaculum spp. as Particularly Active Glucose Assimilators in a Complex Murine Microbiota Cultured In Vitro. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1134219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134219