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Stattic Enhances Radiosensitivity and Reduces Radio-Induced Migration and Invasion in HCC Cell Lines through an Apoptosis Pathway
Joint Authors
Wu, Chaoyang
Xu, Gang
Zhu, Lihua
Wang, Yan
Shi, Yawei
Gong, Aihua
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
Signal transducer and activator of transcription factor 3 (STAT3) is involved in tumorigenesis, development, and radioresistance of many solid tumors.
The aim of this study is to investigate the effects of stattic (an inhibitor of STAT3) on the radiosensitivity and radio-induced migration and invasion ability in hepatocellular carcinoma (HCC) cell lines.
Methods.
HCC cells were treated with stattic, and cell survival rate was analyzed through CCK-8 assay.
Radiosensitivity was evaluated using cloning formation analysis; STAT3, p-STAT3, and apoptosis related proteins were detected by western blot.
Radio-induced migration and invasion ability in HCC cells were analyzed by wound-healing assay and transwell test.
Results.
Stattic inhibits the expression of p-STAT3 and reduces cell survival in a dose-dependent manner in HCC cell lines, and the IC50 values for Hep G2, Bel-7402, and SMMC-7721 are 2.94 μM, 2.5 μM, and 5.1 μM, respectively.
Cloning formation analysis shows that stattic enhances the radiosensitivity of HCC cells.
Wound-healing assay and transwell test show that stattic inhibits radio-induced migration and invasion.
Further study indicates that stattic promotes radio-induce apoptosis through regulating the expression of apoptosis related proteins in HCC cells.
Conclusion.
Stattic enhances radiosensitivity and reduces radio-induced migration and invasion ability in HCC cells probably through apoptosis pathway.
American Psychological Association (APA)
Xu, Gang& Zhu, Lihua& Wang, Yan& Shi, Yawei& Gong, Aihua& Wu, Chaoyang. 2017. Stattic Enhances Radiosensitivity and Reduces Radio-Induced Migration and Invasion in HCC Cell Lines through an Apoptosis Pathway. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1134225
Modern Language Association (MLA)
Xu, Gang…[et al.]. Stattic Enhances Radiosensitivity and Reduces Radio-Induced Migration and Invasion in HCC Cell Lines through an Apoptosis Pathway. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1134225
American Medical Association (AMA)
Xu, Gang& Zhu, Lihua& Wang, Yan& Shi, Yawei& Gong, Aihua& Wu, Chaoyang. Stattic Enhances Radiosensitivity and Reduces Radio-Induced Migration and Invasion in HCC Cell Lines through an Apoptosis Pathway. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1134225
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134225