Fast Localization and Sectioning of Mouse Locus Coeruleus
Joint Authors
Cao, Song
Wang, Zhenglong
Li, Juan
Zhang, Dexin
Yu, Tian
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-02
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The locus nucleus (LC) is a multifunctional nucleus which is also the source of norepinephrine in the brain.
To date, there is no simple and easy method to locate the small LC in brain sectioning.
Here we report a fast, accurate, and easy-to-follow protocol for the localization of mice LC in frozen sectioning.
After fixation and dehydration, the intact brains of adult mice were placed on a horizontal surface and vertically cut along the posterior margin of the bilateral cerebral cortex.
In the coronal cutting plane, the aqueduct of midbrain can be seen easily with the naked eyes.
After embedding the cerebellum part with optimal cutting temperature (OCT) compound, coronal brain slices were cut from the cutting plane, within 1 mm, the aqueduct of midbrain disappeared and the fourth ventricle appeared, then the brain slices contained LC and were collected.
From the first collection, at ~200 μm, the noradrenergic neurons’ most enriched brain slices can be collected.
The tyrosine hydroxylase immunofluorescence staining confirmed that the localization of LC with this method is accurate and the noradrenergic neuron most abundant slices can be determined with this method.
American Psychological Association (APA)
Cao, Song& Li, Juan& Wang, Zhenglong& Zhang, Dexin& Yu, Tian. 2020. Fast Localization and Sectioning of Mouse Locus Coeruleus. BioMed Research International،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1134271
Modern Language Association (MLA)
Cao, Song…[et al.]. Fast Localization and Sectioning of Mouse Locus Coeruleus. BioMed Research International No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1134271
American Medical Association (AMA)
Cao, Song& Li, Juan& Wang, Zhenglong& Zhang, Dexin& Yu, Tian. Fast Localization and Sectioning of Mouse Locus Coeruleus. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1134271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134271