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Protective Effect of a Polyherbal Aqueous Extract Comprised of Nigella sativa (Seeds), Hemidesmus indicus (Roots), and Smilax glabra (Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes
Joint Authors
Thabrew, M. Ira
Samarakoon, Sameera R.
Galhena, Bandula Prasanna
Paul, Solomon F. D.
Perumal, Venkatachalam
Mani, Chinnadurai
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study was carried out to determine the chemoprotective potential of a polyherbal aqueous decoction comprised of Nigella sativa (seeds), Hemidesmus indicus (roots), and Smilax glabra (rhizome) against bleomycin induced cytogenetic damage in human lymphocytes.
Isolated peripheral blood lymphocytes (PBLs) were exposed to bleomycin at a dose of 40 µg/mL for 2 hrs in the presence or absence of different doses of the decoction (100, 300, and 600 µg/mL).
Modulatory effect of the decoction on bleomycin induced cytogenetic damage was evaluated by (a) degree of chromosomal aberrations (CA), (b) formation of micronuclei (MN), and (c) induction of γH2AX foci in lymphocytes exposed to bleomycin.
Lymphocytes pretreated with the decoction showed that a significant reduction (p<0.05) in bleomycin induced (a) stable and unstable chromosome aberrations (CA), (b) MN formation, and (c) formation of γH2AX foci, when compared to lymphocytes treated only with bleomycin.
The decoction by itself did not induce any significant cytogenetic damage in PBLs.
Overall results of the present study confirm that the decoction can attenuate the cytogenetic damage mediated by bleomycin in human PBLs.
American Psychological Association (APA)
Galhena, Bandula Prasanna& Samarakoon, Sameera R.& Thabrew, M. Ira& Paul, Solomon F. D.& Perumal, Venkatachalam& Mani, Chinnadurai. 2017. Protective Effect of a Polyherbal Aqueous Extract Comprised of Nigella sativa (Seeds), Hemidesmus indicus (Roots), and Smilax glabra (Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1134285
Modern Language Association (MLA)
Galhena, Bandula Prasanna…[et al.]. Protective Effect of a Polyherbal Aqueous Extract Comprised of Nigella sativa (Seeds), Hemidesmus indicus (Roots), and Smilax glabra (Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1134285
American Medical Association (AMA)
Galhena, Bandula Prasanna& Samarakoon, Sameera R.& Thabrew, M. Ira& Paul, Solomon F. D.& Perumal, Venkatachalam& Mani, Chinnadurai. Protective Effect of a Polyherbal Aqueous Extract Comprised of Nigella sativa (Seeds), Hemidesmus indicus (Roots), and Smilax glabra (Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1134285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134285