![](/images/graphics-bg.png)
Investigation of Potential Genetic Biomarkers and Molecular Mechanism of Ulcerative Colitis Utilizing Bioinformatics Analysis
Joint Authors
Tong, Xu-Dong
Zhang, Jiaqi
Wang, Xue
Xu, Lin
Zhang, Zedan
Wang, Fengyun
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objectives.
To reveal the molecular mechanisms of ulcerative colitis (UC) and provide potential biomarkers for UC gene therapy.
Methods.
We downloaded the GSE87473 microarray dataset from the Gene Expression Omnibus (GEO) and identified the differentially expressed genes (DEGs) between UC samples and normal samples.
Then, a module partition analysis was performed based on a weighted gene coexpression network analysis (WGCNA), followed by pathway and functional enrichment analyses.
Furthermore, we investigated the hub genes.
At last, data validation was performed to ensure the reliability of the hub genes.
Results.
Between the UC group and normal group, 988 DEGs were investigated.
The DEGs were clustered into 5 modules using WGCNA.
These DEGs were mainly enriched in functions such as the immune response, the inflammatory response, and chemotaxis, and they were mainly enriched in KEGG pathways such as the cytokine-cytokine receptor interaction, chemokine signaling pathway, and complement and coagulation cascades.
The hub genes, including dual oxidase maturation factor 2 (DUOXA2), serum amyloid A (SAA) 1 and SAA2, TNFAIP3-interacting protein 3 (TNIP3), C-X-C motif chemokine (CXCL1), solute carrier family 6 member 14 (SLC6A14), and complement decay-accelerating factor (CD antigen CD55), were revealed as potential tissue biomarkers for UC diagnosis or treatment.
Conclusions.
This study provides supportive evidence that DUOXA2, A-SAA, TNIP3, CXCL1, SLC6A14, and CD55 might be used as potential biomarkers for tissue biopsy of UC, especially SLC6A14 and DUOXA2, which may be new targets for UC gene therapy.
Moreover, the DUOX2/DUOXA2 and CXCL1/CXCR2 pathways might play an important role in the progression of UC through the chemokine signaling pathway and inflammatory response.
American Psychological Association (APA)
Zhang, Jiaqi& Wang, Xue& Xu, Lin& Zhang, Zedan& Wang, Fengyun& Tong, Xu-Dong. 2020. Investigation of Potential Genetic Biomarkers and Molecular Mechanism of Ulcerative Colitis Utilizing Bioinformatics Analysis. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134306
Modern Language Association (MLA)
Zhang, Jiaqi…[et al.]. Investigation of Potential Genetic Biomarkers and Molecular Mechanism of Ulcerative Colitis Utilizing Bioinformatics Analysis. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1134306
American Medical Association (AMA)
Zhang, Jiaqi& Wang, Xue& Xu, Lin& Zhang, Zedan& Wang, Fengyun& Tong, Xu-Dong. Investigation of Potential Genetic Biomarkers and Molecular Mechanism of Ulcerative Colitis Utilizing Bioinformatics Analysis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134306
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134306